Antidiabetic activity of Jering skin extract (Pithecellobium jiringa) with dichloromethane (DCM) solvent fraction

Abstract

Jering skin is a solid waste that can cause problems to the environment if it is not treated seriously. Based on previous research, Jering skin contains highly flavonoids and polyphenols source which potentially used as an antiseptic and anticancer agent. The study aims to evaluate Jering skin antidiabetic activities using varieties DCM solvent fraction. The microplate reader analysis at 410 nm wavelength was used to measure an absorbance level of the Inhibitor Concentration 50 (IC50) sample extracts which inhibited by the α-glucosidase enzyme when hydrolyzing the p-nitrophenyl substrate α-D-glucopyranoside (p-NPG) to form a glucose molecules. As a result, the Jering skin extract is shown to be an alternative antidiabetic agent in the future, with the IC50 level at 417.381 µg/mL.