Evaluation and Optimization of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Salmonella enterica serovar Typhi Isolated from Food Samples

Abstract

CITATION LINKS A R T I C L E I N F O Article Type Original Research Authors Fatemeh Forohi, PhD1* Hadi Zamani, PhD1 Sajjad Yazdansetad, PhD2 How to cite this article Forohi F., Zamani H., Yazdansetad S. Evaluation and Optimization of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Salmonella enterica serovar Typhi Isolated from Food Samples. Infection Epidemiology and Microbiology. 2020;6(4): 259-267 1Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran 2Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran * Correspondence Address: Department of Microbiology, Faculty of Basic Sciences, Shahre-Qods-Branch, Islamic Azad University, Tehran, Iran. f_foroohi@ymail.com Article History Received: July 21 ,2020 Accepted: October 15 ,2020 Published: November 19 ,2020 [1] Bhandari DP. Characterization of flagellin antigen using a panel of ...[2] Babar A, Butt SJ, Ullah S. A review of prognostic models on diagnosis and ... [3] Olobatoke R. Public health burden of non-typhoidal Salmonella strains in sub-Saharan ... [4] Yang Q, Domesle KJ, Wang F, Ge B. Rapid detection of Salmonella in food and feed ... [5] Foddai AC, Grant IR. Methods for detection of viable foodborne pathogens: Current state-of-art ... [6] Zhai L, Liu H, Chen Q, Lu Z, Zhang C, Lv F, et al. Development of a real-time nucleic acid ... [7] Linh Quyen T, Nordentoft S, Chidambara Vinayaka A, Ngo TA, Engelsmenn P, Sun Y, et al. A sensitive, specific, and ... [8] Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, et al. Loop-mediated ... [9] HaraKudo Y, Yoshino M, Kojima T, Ikedo M. Loop-mediated isothermal amplification for ... [10] Havelaar A, Grace D, Wu F. Foodborne diseases from dairy products in developing ...[11] Shahbazi S, Karam MRA, Habibi M, Talebi A, Bouzari S. Distribution of ... [12] Nair A, Balasaravanan T, Malik SS, Mohan V, Kumar M, Vergis J, et al. Isolation and ... [13] Köchl S, Niederstätter H, Parson W. DNA extraction and quantitation of forensic ... [14] Vickers NJ. Animal communication: When I’m calling you, will you answer ... [15] Zhang G, Brown EW, González-Escalona N. Comparison of real-time PCR, reverse transcriptase real-time PCR, loop-mediated isothermal amplification, and the FDA conventional microbiological method for ...[16] Srisawat M, Panbangred W. Efficient and specific detection of Salmonella in food ... [17] Yang Q, Domesle KJ, Ge B. Loop-mediated isothermal amplification for Salmonella detection ... [18] Ravan H, Amandadi M. A rapid and specific detection of pathogenic serovar Salmonella ... [19] Moganedi K, Goyvaerts E, Venter SN, Sibara M. Optimisation of the PCR-invA primers for ... [20] Techathuvanan C, Draughon FA, D’Souza DH. Loop-mediated isothermal amplification (LAMP) for ... [21] Zhao X, Wang L, Chu J, Li Y, Li Y, Xu Z, et al. Development and application of a rapid and simple ... [22] Zhang Y, Shan X, Shi L, Lu X, Tang S, Wang Y, et al. Development of a fimY-based loop-mediated isothermal ...[23] Wang L, Shi L, Alam M, Geng Y, Li L. Specific and rapid detection of foodborne Salmonella by loop-mediated isothermal amplification method. Food Res Int. 2008;41(1):69-74. [24] Chen Z, Zhang K, Yin H, Li Q, Wang L, Liu Z. Detection of Salmonella and several common Salmonella serotypes in food by loop-mediated isothermal amplification method. FSHW. 2015;4(2):75-9. [25] Nagamine K, Hase T, Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Pro Background: Foodborne diseases caused by Salmonella are considered as a global health concern, especially in low-income countries. Rapid and specific detection of this infective agent is highly important in the outbreak control. The current study aimed to design and optimize a LAMP method and to compare its sensitivity and efficiency with the PCR method in the detection of S. typhi in food. Materials & Methods: Food samples including mayonnaise and vegetable salad were inoculated with S. enterica serovar Typhi. Sensitivity and detection limit of LAMP test were investigated at different concentrations of contaminated mayonnaise and vegetable salad. invA gene was chosen as the target gene for bacterial detection by PCR and LAMP tests. Findings: The detection limit of Samonella was estimated to be 16 CFU/mL using LAMP and PCR. LAMP reaction revealed a visible turbidity, indicating the accurate amplification of the selected target gene and proper identification of Salmonella at different dilutions of the studied food samples. Conclusion: The present study indicated that LAMP is a rapid, cost-effective, and specific technique for the identification of Salmonella. This method could be used in laboratories with minimal equipment without the need for costly molecular detection methods.