Extraction, Physico-Chemical Analysis and Microbial Activities Evaluation of the Body Lipid of Tripletail (Lobotes surinamensis) of the Bay of Bengal

Md. Rashedul Azim, A. Afrin, Md. Moazzam Hossain, Sanjida Mukut, M. Helal Uddin
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Abstract

: Due to the discernible variation in the marine fish lipids compositions, the direct and indirect outcomes of it on human health physiology and nutrition have emphasized heightened focus. For this, the inquisition was reported with the extraction of the body lipid of Tripletail ( Lobotes surinamensis ) of the Bay of Bengal by Bligh and Dyer extraction method and different analytical parameters were rummaged and assimilate with those of typical fish lipids. The presence of several fatty acids namely myristic acid (C14:0), palmitic acid (C16:0), stearic acid (C18:0), palmitoleic acid (C16:1), oleic acid (C18:1), vaccenic acid (C18:1), linoleic acid (C18:2), linolenic acid (C18:3), arachidonic acid (C20:4), eicosadienoic acid (C20:2), eicosapentaenoic acid (C20:5), docosapentaenoic acid (C22:5) and docosahexaenoic acid (C22:6) etc was confirmed by Gas-Liquid Chromatography (GLC). It revealed that Saturated Fatty Acids (SFA%) were higher than Unsaturated Fatty Acids (UFA%). Moreover total Monounsaturated Fatty Acids (MUFA%) were higher than Polyunsaturated Fatty Acids (PUFA%) i.e. [25.5777% > 12.6850%] and ω-3 Polyunsaturated Fatty Acids (ω-3 PUFA) were higher than ω-6 Polyunsaturated Fatty Acids (ω-6 PUFA) i.e. [8.1113% > 4.5737%] along with higher ω-3/ω-6 ratio (1.77). The muscle of the target specimen was assessed to quantify the minerals content (N, P, K and Ca). Several metals (Fe, Pb, Ni, Co, Cd, Cu, Zn, Mn, Cr, As, Mg) were estimated with the help of Atomic Absorption Spectrophotometric (AAS) method. Additionally, using established techniques, the isolated lipid sample was evaluated for microbial (bacterial and fungal) activity. Thus from the findings, it is resolved with a variety of significant facts relating to nutritional and medicinal aspects.
孟加拉湾三尾鱼体脂的提取、理化分析及微生物活性评价
由于海洋鱼类的脂类成分存在明显的差异,其对人体健康、生理和营养的直接和间接影响已引起人们的高度关注。为此,采用Bligh - Dyer法对孟加拉湾三尾鱼体脂进行了提取,并对不同的分析参数进行了整理,与典型鱼类脂质进行了同化。气液色谱法(GLC)证实了几种脂肪酸的存在:肉豆酸(C14:0)、棕榈酸(C16:0)、硬脂酸(C18:0)、棕榈油酸(C16:1)、油酸(C18:1)、异丙酸(C18:1)、亚油酸(C18:2)、亚麻酸(C18:3)、花生四烯酸(C20:4)、二十碳二烯酸(C20:2)、二十碳五烯酸(C20:5)、二十二碳六烯酸(C22:5)、二十二碳六烯酸(C22:6)等。结果表明,饱和脂肪酸(SFA%)高于不饱和脂肪酸(UFA%)。总单不饱和脂肪酸(MUFA%)高于多不饱和脂肪酸(PUFA%),即[25.5777% > 12.6850%];ω-3多不饱和脂肪酸(ω-3 PUFA)高于ω-6多不饱和脂肪酸(ω-6 PUFA),即[8.1113% > 4.5737%],且ω-3/ω-6比值较高(1.77)。评估目标标本的肌肉以量化矿物质含量(N, P, K和Ca)。利用原子吸收分光光度法(AAS)对几种金属(Fe, Pb, Ni, Co, Cd, Cu, Zn, Mn, Cr, As, Mg)进行了测定。此外,使用已建立的技术,对分离的脂质样品进行微生物(细菌和真菌)活性评估。因此,从研究结果来看,它与营养和医学方面的各种重要事实有关。
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