Molecular confirmation of twinning in the West Indian Manatee (Trichechus manatus)

IF 1 4区环境科学与生态学 Q3 BIODIVERSITY CONSERVATION

Biota Neotropica Pub Date : 2022-01-01 DOI:10.1590/1676-0611-bn-2021-1241

Sávia Moreira, A. Meirelles, V. Carvalho, P. Rêgo, J. Araripe

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引用次数: 1

Abstract

Abstract: Few twinning events have been recorded in the West Indian manatee (Trichechus manatus, Sirenia: Trichechidae) and no previous published study has provided confirmation of this phenomenon based in molecular tools. Here we investigate a possible case of twinning in an endangered Brazilian population of T. manatus using molecular tools. We analyzed two male neonates found stranded in Ceará State, on the northeastern coast of Brazil. The DNA of both individuals was isolated, and 10 microsatellite loci were amplified and genotyped. Following the identification of the alleles, the probabilities of identity by descent (∆7 and ∆8) and relatedness (rxy) were calculated using estimators that evaluate inbreeding. The two individuals shared most of the alleles, with differences in the genotypes being identified in only two loci. All the estimators identified a level of relatedness compatible with that found between siblings (selfed or outbred), indicating they were dizygotic twins. This is the first confirmed case of fraternal twins in free-ranging West Indian manatees in South America. The recognition of this type of twinning provides elements to improve actions for the rehabilitation of stranded animals and their subsequent release to the environment.

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西印度海牛(trichecchus manatus)双胞胎的分子确证

摘要:在西印度海牛(Trichechus manatus, Sirenia: Trichechidae)中很少有双胞胎事件的记录，以前没有发表的基于分子工具的研究证实这一现象。在这里，我们调查了一个可能的孪生案例，在一个濒临灭绝的巴西种群使用分子工具。我们分析了两只被困在巴西东北海岸塞埃尔州的雄性新生儿。分离两种个体的DNA，扩增10个微卫星位点并进行基因分型。在确定等位基因后，使用评估近交的估计器计算遗传同一性(∆7和∆8)和亲缘性(rxy)的概率。这两个人共享了大部分等位基因，基因型的差异仅在两个位点上被鉴定出来。所有的估计值都确定了与兄弟姐妹(自交或近亲繁殖)之间的亲缘关系一致的水平，表明他们是异卵双胞胎。这是在南美洲自由放养的西印度海牛中首次确认的异卵双胞胎病例。认识到这种类型的双胞胎为改善搁浅动物的康复行动以及随后将其释放到环境中提供了要素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biota Neotropica BIODIVERSITY CONSERVATION-

CiteScore

2.90

自引率

16.70%

发文量

审稿时长

4-8 weeks

期刊介绍： BIOTA NEOTROPICA is an electronic, peer-reviewed journal edited by the Program BIOTA/FAPESP: The Virtual Institute of Biodiversity. This journal"s aim is to disseminate the results of original research work, associated or not to the program, concerned with characterization, conservation and sustainable use of biodiversity within the Neotropical region. Manuscripts are considered on the understanding that their content has not appeared, or will not be submitted, elsewhere in substantially the same form, because once published their copyrights are transferred to BIOTA NEOTROPICA as established in the Copyright Transfer Agreement signed by the author(s).