Identification of Poly(ADP-ribose) Polymerase 9 (PARP9) as a Potent Suppressor for Mycobacterium tuberculosis Infection.

IF 7.6 1区 农林科学 Q1 AGRONOMY
Plant Phenomics Pub Date : 2023-09-04 eCollection Date: 2024-04-01 DOI:10.1007/s43657-023-00112-2
Zhenyu Zhu, Shufeng Weng, Fen Zheng, Qi Zhao, Ying Xu, Jiaxue Wu
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引用次数: 0

Abstract

ADP-ribosylation is a reversible and dynamic post-translational modification mediated by ADP-ribosyltransferases (ARTs). Poly(ADP-ribose) polymerases (PARPs) are an important family of human ARTs. ADP-ribosylation and PARPs have crucial functions in host-pathogen interaction, especially in viral infections. However, the functions and potential molecular mechanisms of ADP-ribosylation and PARPs in Mycobacterium infection remain unknown. In this study, bioinformatics analysis revealed significantly changed expression levels of several PARPs in tuberculosis patients compared to healthy individuals. Moreover, the expression levels of these PARPs returned to normal following tuberculosis treatment. Then, the changes in the expression levels of PARPs during Mycobacterium infection were validated in Tohoku Hospital Pediatrics-1 (THP1)-induced differentiated macrophages infected with Mycobacterium model strains bacillus Calmette-Guérin (BCG) and in human lung adenocarcinoma A549 cells infected with Mycobacterium smegmatis (Ms), respectively. The mRNA levels of PARP9, PARP10, PARP12, and PARP14 were most significantly increased during infection, with corresponding increases in protein levels, indicating the possible biological functions of these PARPs during Mycobacterium infection. In addition, the biological function of host PARP9 in Mycobacterium infection was further studied. PARP9 deficiency significantly increased the infection efficiency and intracellular proliferation ability of Ms, which was reversed by the reconstruction of PARP9. Collectively, this study updates the understanding of changes in PARP expression during Mycobacterium infection and provides evidence supporting PARP9 as a potent suppressor for Mycobacterium infection.

Supplementary information: The online version contains supplementary material available at 10.1007/s43657-023-00112-2.

鉴定聚(ADP-核糖)聚合酶 9 (PARP9) 是结核分枝杆菌感染的强效抑制因子
ADP-ribosylation 是由 ADP-ribosyltransferases (ARTs) 介导的一种可逆的动态翻译后修饰。聚(ADP-核糖)聚合酶(PARPs)是人类 ARTs 的一个重要家族。ADP-ribosylation 和 PARPs 在宿主与病原体的相互作用中,尤其是在病毒感染中具有重要功能。然而,ADP-核糖基化和 PARPs 在分枝杆菌感染中的功能和潜在分子机制仍然未知。本研究通过生物信息学分析发现,与健康人相比,结核病患者体内几种 PARPs 的表达水平发生了显著变化。此外,这些 PARPs 的表达水平在结核病治疗后恢复正常。随后,研究人员分别在东北医院儿科-1(THP1)诱导的分化巨噬细胞感染分枝杆菌模型菌株卡介苗(BCG)和人肺腺癌A549细胞感染分枝杆菌(Ms)后,验证了分枝杆菌感染过程中PARPs表达水平的变化。在感染过程中,PARP9、PARP10、PARP12和PARP14的mRNA水平明显升高,蛋白水平也相应升高,这表明这些PARPs在分枝杆菌感染过程中可能具有生物学功能。此外,还进一步研究了宿主 PARP9 在分枝杆菌感染中的生物学功能。PARP9的缺失会明显提高Ms的感染效率和细胞内增殖能力,而PARP9的重建会逆转这种情况。总之,这项研究更新了人们对分枝杆菌感染过程中PARP表达变化的认识,并提供了支持PARP9作为分枝杆菌感染强效抑制因子的证据:在线版本包含补充材料,可查阅 10.1007/s43657-023-00112-2。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant Phenomics
Plant Phenomics Multiple-
CiteScore
8.60
自引率
9.20%
发文量
26
审稿时长
14 weeks
期刊介绍: Plant Phenomics is an Open Access journal published in affiliation with the State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agricultural University (NAU) and published by the American Association for the Advancement of Science (AAAS). Like all partners participating in the Science Partner Journal program, Plant Phenomics is editorially independent from the Science family of journals. The mission of Plant Phenomics is to publish novel research that will advance all aspects of plant phenotyping from the cell to the plant population levels using innovative combinations of sensor systems and data analytics. Plant Phenomics aims also to connect phenomics to other science domains, such as genomics, genetics, physiology, molecular biology, bioinformatics, statistics, mathematics, and computer sciences. Plant Phenomics should thus contribute to advance plant sciences and agriculture/forestry/horticulture by addressing key scientific challenges in the area of plant phenomics. The scope of the journal covers the latest technologies in plant phenotyping for data acquisition, data management, data interpretation, modeling, and their practical applications for crop cultivation, plant breeding, forestry, horticulture, ecology, and other plant-related domains.
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