Study of Rubus Idaeus Juice Ellagitannins and Its Antimicrobial Activity

Abstract

Medicines on the basis of herbs are increasingly used for treating of many diseases, providing the following actions antimicrobial, antiviral, anticancer, anti-enzymatic and immunomodulatory. Ellagitannins are a group of polyphenolic compounds of plant origin, which exhibiting a strong antibacterial effect in virtue of their ability to form a bond with the microbial membrane. Raspberry contains a large amount of ellagitannins, so it is advisable to study their composition and content in raspberry juice, obtained in Ukraine and determine its antimicrobial activity. As raw material Ukrainian variety ‘Phenomenon’ was taken for the research. It was bred in 1991 in Kharkiv region by crossing the varieties ‘Stolichnaya’ and ‘Odarka’. Analysis of raspberry juice ellagitannins was performed by HPLC method. 4 ellagic acid derivatives were identified and their contents were established. Unbound ellagic acid (55% of all) and sanguiin H-6 (21% of all) had the highest content among them Antimicrobial activity of raspberry juice was studied by agar diffusion method. The juice showed antimicrobial activity against Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Bacillus subtilis and Candida albicans. The antimicrobial activity of the juice was higher than the comparison drug " Chlorophyllipt " for more than twice in relation to all strains. The greatest effect was manifested relative to Staphylococcus aureus, which can be the cause of purulent-inflammatory processes in almost all human organs with severe consequences. This is especially true in connection with the increasing resistance of strains not only to traditional antibiotics, but also to new groups.