Determination of Efficacy of Allium sativum (Garlic) Bulbs Extracts on Some Enteric (Pathogenic) Bacteria

T. Matthew
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Abstract

This study was carried out to determine whether ethanol and aqueous extracts of Allium sativum (garlic) could effectively eliminate pure cultures of selected enteric and pathogenic bacteria. Ethanol and water extracts of Allium sativum (garlic) bulbs were made through cold extraction and then concentrated by refluxing in soxhlet apparatus. A crude (raw) extract of the bulbs was also obtained by blending and then using muslin cloth to squeeze the juice out. These extracts were tried at various concentrations on four species of enteric bacteria namely: Salmonella typhi, Salmonella paratyphi, Pseudomonas aeruginosa and Klebsiella spp. to observe their efficacy on these pathogenic bacteria. Crude extract showed a highly positive result and the rest were negative.
大蒜球茎提取物对某些肠道(致病菌)细菌药效的测定
本研究旨在确定大蒜的乙醇和水提物是否能有效地清除选定肠道病原菌和致病菌的纯培养物。采用冷浸法制备大蒜球茎的乙醇和水提取物,然后在索氏装置中回流浓缩。通过混合,然后用细布把汁液挤出来,也可以得到一种粗的(未加工的)球茎提取物。以不同浓度对伤寒沙门菌、副伤寒沙门菌、铜绿假单胞菌和克雷伯氏菌4种肠道细菌进行抑菌试验,观察其抑菌效果。粗提物呈高度阳性,其余为阴性。
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