Anti microbial effect of prebiotic containing tooth paste against streptococcus mutans and lactobacillus: An invitro evaluation

S. R, Preetha Peethambar, S. Konde, M. Agarwal, Sahana N Prasad
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Abstract

: To evaluate the antimicrobial efficacy of three commercially available toothpastes which are non-fluoridated, fluoridated and the one containing prebiotic ingredient on s and .: Antimicrobial activities of prebiotic, fluoridated, non-fluoridated toothpastes were assessed at different concentrations (100ug, 200ug, 300ug and 400ug). Since prebiotic toothpaste had other proven antimicrobial agents, 100% inulin extract from chicory (prebiotic) was assessed for its antimicrobial efficacy at varying concentrations. Antimicrobial property was evaluated by determining the zone of inhibition using agar well diffusion method on Luria Bertani (LB) broth media for and MRS broth media forL.plantarum. 100ul of inoculums of S.mutans and L.plantarum was poured on to the agar plates respectively. Five wells measuring 0.5cm was made, dentifrice at varying concentration i.e; 100ug, 200ug, 300ug and 400ug was loaded into respective wells. 50ul of DMSO was loaded in the well at the centre as control and incubated at 37°C for 24hrs. : All the three tested toothpastes had significant antimicrobial activity against S.mutans and L.plantarum. The highest antimicrobial activity was exhibited by toothpaste containing prebiotic ingredient with mean zone of inhibition of (18.5+/-0.5) and (20.0+/-1) at 400ug against S.mutans and L.plantarum. There was no significant difference in antimicrobial activity of fluoridated and non-fluoridated toothpastes against S.mutans at 400ug (P=1), but there was a significant difference for L.plantarum at 400ug (P<0.001). Antimicrobial activity increased at higher concentrations for all the three tested toothpastes and for 100% Inulin extract. : All the three tested toothpaste were effective against S.mutans and L.plantarum at varying concentrations, but toothpaste containing prebiotic ingredient was most effective in inhibition of both the micro-organisms.
含益生元牙膏对变形链球菌和乳酸菌的抑菌作用:体外评价
为了评估三种市售牙膏的抗菌效果,分别是不含氟牙膏、含氟牙膏和含益生元成分牙膏。在不同浓度(100ug、200ug、300ug和400ug)下,对益生元牙膏、含氟牙膏和不含氟牙膏的抗菌活性进行了评估。由于益生元牙膏含有其他已证实的抗菌剂,因此对菊苣(益生元)100%菊粉提取物在不同浓度下的抗菌效果进行了评估。采用琼脂孔扩散法对植物褐藻(Luria Bertani, LB)肉汤培养基和MRS肉汤培养基进行抑菌区鉴定。分别将变形链球菌和植物芽孢杆菌接种体100ul倒在琼脂板上。制作5个孔,孔长0.5cm,分别取不同浓度的牙液;分别入井100ug、200ug、300ug、400ug。将50ul DMSO装入中心孔中作为对照,在37℃条件下孵育24h。三种牙膏对变形链球菌和植物乳杆菌均有显著的抑菌活性。含益生元成分的牙膏在400ug时对变形链球菌和植物l.a tarum的平均抑制区分别为(18.5+/-0.5)和(20.0+/-1),抗菌活性最高。含氟牙膏与非含氟牙膏在400ug浓度下对变形链球菌的抑菌活性差异无统计学意义(P=1),而对植物乳杆菌的抑菌活性差异有统计学意义(P<0.001)。三种牙膏和100%菊粉提取物浓度越高,抗菌活性越强。三种牙膏在不同浓度下均能有效抑制变形链球菌和植物乳杆菌,但含益生元成分的牙膏对这两种微生物的抑制效果最好。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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