INVESTIGATING THE EFFECT OF COLD TEMPERATURE STRESS ON UNOPENED MALE CATKINS AND INOCULATED FEMALE FLOWERS OF IRANIAN NATIVE HAZELNUT CULTIVARS

Abstract

In many low-temperature areas, the environmental factor is an important limiting factor for the production and distribution of horticultural plants. This study aimed to investigate the cold tolerance of the male catkins and inoculated female flowers to screen the popular native hazelnut cultivars in Qazvin under low-temperature stress. A completely randomized factorial block design with three replications was used in this experiment with eight cultivars (Nakhnroud, Khandan, Mish-Pestan, South of Qarabagh, Asl-e-Qarabagh, Rasmi, and Gerdashkevar). After removing each of the treated samples at the end of the experiment, the samples were examined morphologically (appearance) and compared with the control. The changes were recorded as qualitative traits. To understand the influence of cold stress on reproductive organs, hydrogen peroxide and proline were measured. The results showed the onset of freezing in unopened male catkins at -7 and -9 °C and in inoculated female flowers at -3 °C. Damage to unopened male catkins' tissue occurred at -11 °C and in female flowers at -5 °C. The highest value observed among cultivars in the case for proline content of male catkins was in Mish-Pestan and Khandan cultivars with 0.816 and 0.660 µmol/ g FW, respectively. In inoculated female flowers, Mish-Pestan and Tabestaneh cultivars with 0.185 and 0.168 µmol/ g FW, respectively, showed the highest statistically significant increase in proline content. Interestingly, the cultivars with the highest proline content in male catkins indicated the most increase in H2O2; Mish-Pestan and Khnadan with 0.569 and 0.541 ug/g FW, respectively. Asl-eQarabagh was observed to have the least H2O2 content (0.042 ug/g FW) among cultivars. Again, in inoculated female flowers, those with the highest concentration of proline (Mish-Pestan and Tabestaneh) were found to have the highest H2O2 content (0.335 and 0.331 ug/g FW, respectively