Morphology and Molecular Studies and Differentiation Ability of Ovarian Stem Cells in Newborn Syrian Rats

S. Adib
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Abstract

Background and Objectives: The ability of ovarian stem cells (OSCs) to differentiate into oocyte-like and theca cells may lead to major advances in infertility treatment. However, little is known about the function, growth and differentiation potentials of these cells. In this study, we aim to examine the characteristics of OSCs obtained from newborn rats and assess their ability to differentiate into osteocyte-like and adipocyte-like cells. Methods: In this study, OSCs were isolated from newborn Syrian rats and were then cultured. After the third passage, using the PCR method, the expression of stem cell markers (Sox2, Pou5f, and Nanog) was examined. Their potential to differentiate into osteocyte-like and adipocyte-like cells was examined by Alizarin red and Oil red staining, respectively. Results: The isolated OSCs were adherent and morphologically similar to fibroblast cells, and had the power to multiply. They expressed stem cells markers. In addition, they were able to differentiate into adipocyte-like and osteocyte-like cells 21 days after being placed in the differentiation medium. In the osteocyte-like cells, fat droplets were observed after oil red staining. Conclusion: The OSCs include multipotent stem cells that are able to express markers related to stem cells and can differentiate into osteocyte-like and adipocyte-like cells.
新生叙利亚大鼠卵巢干细胞的形态分子研究及分化能力
背景和目的:卵巢干细胞(OSCs)分化为卵母细胞样细胞和卵泡细胞的能力可能导致不孕症治疗的重大进展。然而,人们对这些细胞的功能、生长和分化潜能知之甚少。在这项研究中,我们的目的是研究新生大鼠获得的osc的特征,并评估它们向骨细胞样细胞和脂肪细胞样细胞分化的能力。方法:本研究从新生叙利亚大鼠中分离osc并进行培养。第三代后,采用PCR方法检测干细胞标记物(Sox2, Pou5f, Nanog)的表达。分别用茜素红和油红染色检测其向骨细胞样细胞和脂肪细胞样细胞分化的潜力。结果:分离的OSCs具有粘附性,形态与成纤维细胞相似,具有增殖能力。它们表达了干细胞标记物。此外,它们在分化培养基中放置21天后能够分化为脂肪细胞样细胞和骨细胞样细胞。在骨细胞样细胞中,油红染色可见脂肪滴。结论:OSCs包括多能干细胞,能够表达与干细胞相关的标志物,并能分化为骨细胞样细胞和脂肪细胞样细胞。
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