Jiun-Yan Wu , Jenn-Wen Huang , Hsin-Der Shih , Wei-Chen Lin , Yung-Chuan Liu
{"title":"Optimization of cultivation conditions for fungichromin production from Streptomyces padanus PMS-702","authors":"Jiun-Yan Wu , Jenn-Wen Huang , Hsin-Der Shih , Wei-Chen Lin , Yung-Chuan Liu","doi":"10.1016/j.jcice.2007.11.006","DOIUrl":null,"url":null,"abstract":"<div><p>A novel strain, <em>Streptomyces padanus</em> PMS-702, was employed to produce fungichromin (FC, a polyene macrolide antibiotic) in a shake flask cultivation. In comparing the effects of various carbon and nitrogen sources on PMS-702 cultivation, it was found that glucose and soybean meal medium yielded the highest FC within 2 days. Factors such as medium composition, cultivation temperatures, and initial pH were optimized for FC production with response surface methodology (RSM). The optimal cultural condition obtained is as follows: glucose 11.2<!--> <!-->g/L, soybean meal 11.2<!--> <!-->g/L, CaCO<sub>3</sub> 0.46<!--> <!-->g/L, temperature 31.7<!--> <!-->°C, and an initial pH 5.5. Under these conditions, FC production reached 112<!--> <!-->mg/L, about an increase of 2.86 times, as compared to results under basic conditions.</p></div>","PeriodicalId":17285,"journal":{"name":"Journal of The Chinese Institute of Chemical Engineers","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jcice.2007.11.006","citationCount":"26","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of The Chinese Institute of Chemical Engineers","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0368165307001232","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 26
Abstract
A novel strain, Streptomyces padanus PMS-702, was employed to produce fungichromin (FC, a polyene macrolide antibiotic) in a shake flask cultivation. In comparing the effects of various carbon and nitrogen sources on PMS-702 cultivation, it was found that glucose and soybean meal medium yielded the highest FC within 2 days. Factors such as medium composition, cultivation temperatures, and initial pH were optimized for FC production with response surface methodology (RSM). The optimal cultural condition obtained is as follows: glucose 11.2 g/L, soybean meal 11.2 g/L, CaCO3 0.46 g/L, temperature 31.7 °C, and an initial pH 5.5. Under these conditions, FC production reached 112 mg/L, about an increase of 2.86 times, as compared to results under basic conditions.
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