Study on the correlation between Pokemon protein and P14ARF-MDM2-p53 pathway proteins in non-small cell lung cancer

Abstract

Objective To investigate the role of Pokemon protein and P14ARF-MDM2-p53 pathway proteins in the pathogenesis of non-small cell lung cancer (NSCLC). Methods This study included 35 NSCLC patients who underwent surgical treatment from the First Hospital of Hebei Medical University from October 2015 to October 2016.At the same time, 29 cases of normal bronchus stump adjacent to the cancer and 25 cases of pneumonia were used as the bronchial control group and alveolar control group, respectively.Immunohistochemistry was used to detect the expressions of Pokemon, P14ARF, MDM2 and p53 proteins in NSCLC group, bronchial control group and alveolar control group, and to analyze their relationship with clinicopathological data and their correlations. Results The expressions of Pokemon, MDM2 and p53 proteins in NSCLC group were significantly higher than those in the bronchial control group and the alveolar control group (all P values <0.01). The expression of P14ARF protein in NSCLC group was significantly lower than that in the bronchial control group and alveolar control group (χ2=25.39, P<0.01). The expressions of p53 and MDM2 protein were correlated with the degree of differentiation of NSCLC (P=0.02, 0.03). In NSCLC group, Pokemon was negatively correlated with P14ARF protein expression (r=-0.58, P<0.05), and P14ARF was negatively correlated with MDM2 protein expression (r=-0.51, P<0.05), MDM2 was positively correlated with p53 protein expression (r=0.86, P<0.05). Conclusions Pokemon plays an oncogene role in the pathogenesis of NSCLC by regulating the P14ARF-MDM2-p53 pathway. Key words: Carcinoma, non-small-cell lung; Immunohistochemistry; Pokemon; P14ARF; MDM2