{"title":"Lactose hydrolysis in milk by immobilized β-galactosidase","authors":"J. Rogalski , A. Dawidowicz , A. Leonowicz","doi":"10.1016/0304-5102(94)00092-1","DOIUrl":null,"url":null,"abstract":"<div><p>The intolerance of a large number of children and adults for the lactose containing products caused a wide spread of investigations of the decrease of this bisaccharide in meals carried out by the application of such enzymes as β-galactosidases. The properties of four different commercial preparations of β-galactosidase originating from bacteria and fungi are described. The native enzymes are compared with their immobilized forms. pH and temperature optimalization and stability curves are presented not only for the immobilized enzymes but also for those which were additionally cross-linked with glutaraldehyde or bis-oxirane. Lactozym 3000 in an immobilized form seems to be the most useful for the delactatic process of commercially available milk.</p></div>","PeriodicalId":16567,"journal":{"name":"分子催化","volume":"93 2","pages":"Pages 233-245"},"PeriodicalIF":0.0000,"publicationDate":"1994-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0304-5102(94)00092-1","citationCount":"19","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"分子催化","FirstCategoryId":"1089","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0304510294000921","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Chemical Engineering","Score":null,"Total":0}
引用次数: 19
Abstract
The intolerance of a large number of children and adults for the lactose containing products caused a wide spread of investigations of the decrease of this bisaccharide in meals carried out by the application of such enzymes as β-galactosidases. The properties of four different commercial preparations of β-galactosidase originating from bacteria and fungi are described. The native enzymes are compared with their immobilized forms. pH and temperature optimalization and stability curves are presented not only for the immobilized enzymes but also for those which were additionally cross-linked with glutaraldehyde or bis-oxirane. Lactozym 3000 in an immobilized form seems to be the most useful for the delactatic process of commercially available milk.
期刊介绍：
Journal of Molecular Catalysis (China) is a bimonthly journal, founded in 1987. It is a bimonthly journal, founded in 1987, sponsored by Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, under the supervision of Chinese Academy of Sciences, and published by Science Publishing House, which is a scholarly journal openly circulated both at home and abroad. The journal mainly reports the latest progress and research results on molecular catalysis. It contains academic papers, research briefs, research reports and progress reviews. The content focuses on coordination catalysis, enzyme catalysis, light-ribbed catalysis, stereochemistry in catalysis, catalytic reaction mechanism and kinetics, the study of catalyst surface states and the application of quantum chemistry in catalysis. We also provide contributions on the activation, deactivation and regeneration of homogeneous catalysts, solidified homogeneous catalysts and solidified enzyme catalysts in industrial catalytic processes, as well as on the optimisation and characterisation of catalysts for new catalytic processes.
The main target readers are scientists and postgraduates working in catalysis in research institutes, industrial and mining enterprises, as well as teachers and students of chemistry and chemical engineering departments in colleges and universities. Contributions from related professionals are welcome.
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