Characterization of colon cancer cells from a patient at Ciptomangun Kusumo National Hospital using CD44, NANOG and OCT4 gene

Abstract

CD44 is a cell adhesion molecule and has been recognized as a cancer marker that plays an important role in tumor progression and metastasis. The role of CD44 in tumorigenesis is due to its binding to extracellular matrix components, including hyaluronan (HA) and osteopontin (OPN) and to messenger molecules, such as growth factors present in the tumor microenvironment where they contribute to the maintenance of the stemness of malignant cells. Nanog and Oct are pluripotent stem cells that play roles in development of cancer stem cell. The aim of this research was to investigate expression of CD44, Nanog and Oct 4 at colorectal patient stadium IV. Biopsy of patient was extracted using collagenese I and incubate at 37°C for 30 minutes. Upon biopsy, the tissues were analyzed using Tali cytometer and indirect immunostaining for CD44, Nanog and OCT 4 expressions at days 3, 7 and 10. The results were compared with that of colo cell line. Results revealed the significance differences in gene expression between samples from patients with line colo cells originating from Caucasian colon cancer patients. Such differences necessitated for more in-depth researches towards the gene expression of Indonesian colon cancer patients that may prove to be useful for developing future anti-cancer drugs.CD44 is a cell adhesion molecule and has been recognized as a cancer marker that plays an important role in tumor progression and metastasis. The role of CD44 in tumorigenesis is due to its binding to extracellular matrix components, including hyaluronan (HA) and osteopontin (OPN) and to messenger molecules, such as growth factors present in the tumor microenvironment where they contribute to the maintenance of the stemness of malignant cells. Nanog and Oct are pluripotent stem cells that play roles in development of cancer stem cell. The aim of this research was to investigate expression of CD44, Nanog and Oct 4 at colorectal patient stadium IV. Biopsy of patient was extracted using collagenese I and incubate at 37°C for 30 minutes. Upon biopsy, the tissues were analyzed using Tali cytometer and indirect immunostaining for CD44, Nanog and OCT 4 expressions at days 3, 7 and 10. The results were compared with that of colo cell line. Results revealed the significance differences in gene expression between s...