Preparation and Evaluation of Antidiabetic Activity of Allium cepa-Phospholipid Complex (Phytosome) in Streptozotocin Induced Diabetic Rats

RGUHS Journal of Pharmaceutical Sciences Pub Date : 2015-01-01 DOI:10.5530/rjps.2015.4.3

P. Habbu, Smita Madagundi, R. Shastry, Rashmi Vanakudri, V. Kulkarni

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引用次数: 8

Abstract

Purpose: A phytoformulation, Allium cepa-phospholipid complex (ACP) was prepared, characterized and evaluated for its antidiabetic activity in Streptozotocin (STZ) induced rats. Methodology: ACP was prepared and characterized by SEM, DSC and FT-IR. It was then screened for in vitro free radical scavenging, antidiabetic activity (50 mg/kg and 100 mg/kg, p.o.) in normoglycemic and STZ induced diabetic animals. Concentration of quercetin in ACP was estimated in serum by HPLC. Findings: SEM data showed that ACP has irregular size vesicles consisting of HSPC and ACE intercalated in the lipid layer. ACP showed one endothermal peak in DSC studies. ACP showed good in vitro antioxidant activity. In OGTT, treatment with ACE (Allium cepa extract), ACP and glibenclamide significantly improved the glucose tolerance in normal animals. In STZ induced diabetic rats, a single dose of ACE and ACP treatment exhibited reduction in SG levels at different time intervals compared to basal levels. Administration of both the doses of ACE and ACP for fifteen days exhibited greater percentage reduction in glycemia and restored to near normal value of all tested lipid parameters. Higher serum concentration of quercetin was observed for ACP in bioavailability studies as compared to ACE. Conclusion: ACP has shown significant antioxidant, antidiabetic activity and hypolipidemic activity in STZ induced rats as compared to ACE at the dose studied.

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葱a磷脂复合物(Phytosome)对链脲佐菌素诱导的糖尿病大鼠抗糖尿病活性的研究

目的:制备葱A磷脂复合物(Allium cepa-磷脂复合物，ACP)，对其在链脲佐菌素(STZ)诱导大鼠体内的抗糖尿病活性进行表征和评价。方法:制备ACP，并采用SEM、DSC、FT-IR对其进行表征。然后对正常血糖和STZ诱导的糖尿病动物进行体外自由基清除和抗糖尿病活性(50 mg/kg和100 mg/kg, p.o.)筛选。采用高效液相色谱法测定血清ACP中槲皮素的含量。结果:扫描电镜显示，ACP有不规则大小的小泡，由HSPC和ACE组成，嵌入脂质层。在DSC研究中，ACP显示一个吸热峰。ACP具有良好的体外抗氧化活性。在OGTT中，用ACE (Allium cepa提取物)、ACP和格列本脲治疗可显著改善正常动物的葡萄糖耐量。在STZ诱导的糖尿病大鼠中，与基础水平相比，单剂量ACE和ACP治疗可在不同时间间隔内降低SG水平。同时给予ACE和ACP 15天，血糖下降的百分比更大，所有测试的血脂参数恢复到接近正常值。在生物利用度研究中，与ACE相比，ACP的血清槲皮素浓度较高。结论:与ACE相比，ACP对STZ诱导的大鼠具有显著的抗氧化、降糖和降血脂活性。

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RGUHS Journal of Pharmaceutical Sciences

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