{"title":"Starfish oocyte maturation: from prophase to metaphase","authors":"Laurent Meijer, Guy Mordret","doi":"10.1006/sedb.1994.1022","DOIUrl":null,"url":null,"abstract":"<div><p>The biochemical pathways underlying the prophase/metaphase transition have been studied extensively in isolated starfish oocytes. These cells are released from their meiotic prophase arrest by 1-methyladenine. This hormone interacts with plasma membrane receptors coupled to heterotrimeric G-proteins. Early events following 1-methyladenine/receptor interaction include a decrease of cAMP concentration and possibly involve tyrosine kinases, phospholipases A2 and C, proteases and phosphatase 2A. Later events include the activation of an M phase-Promoting Factor (MPF) through dephosphorylation/phosphorylation of its p34<sup>cdc2</sup> and cyclin B subunits, MPF translocation to the nucleus and activation of the MAP-kinase p44<sup>mpk</sup>. Starfish oocytes provide an exceptional model to investigate a hormone-regulated cell cycle phase as well as a unique source for the purification of cell cycle control elements.</p></div>","PeriodicalId":101155,"journal":{"name":"Seminars in Developmental Biology","volume":"5 3","pages":"Pages 165-171"},"PeriodicalIF":0.0000,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/sedb.1994.1022","citationCount":"21","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Seminars in Developmental Biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S104457818471022X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 21
Abstract
The biochemical pathways underlying the prophase/metaphase transition have been studied extensively in isolated starfish oocytes. These cells are released from their meiotic prophase arrest by 1-methyladenine. This hormone interacts with plasma membrane receptors coupled to heterotrimeric G-proteins. Early events following 1-methyladenine/receptor interaction include a decrease of cAMP concentration and possibly involve tyrosine kinases, phospholipases A2 and C, proteases and phosphatase 2A. Later events include the activation of an M phase-Promoting Factor (MPF) through dephosphorylation/phosphorylation of its p34cdc2 and cyclin B subunits, MPF translocation to the nucleus and activation of the MAP-kinase p44mpk. Starfish oocytes provide an exceptional model to investigate a hormone-regulated cell cycle phase as well as a unique source for the purification of cell cycle control elements.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
