Variation in Lycopene Epsilon-Cyclase Gene in Selected Tomato Genotypes Revealed by EcoTILLING and Sequencing

Q3 Multidisciplinary
P. Guevarra, Roanne Gardoce, Hayde F. Galvez
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引用次数: 0

Abstract

Selected tomato genotypes with contrasting fruit colors of orange and red were investigated for sequence-level variations of candidate genes involved in lycopene cyclization. Sequence-specific markers for tomato lycopene beta-cyclase (3) and lycopene epsilon-cyclase (1) genes were designed and used to screen for putative single nucleotide polymorphisms (SNPs) through Ecotype Targeted Induced Local Lesions IN Genome (EcoTILLING) and Sanger sequencing. Despite being regarded as among the evolutionarily conserved genes in the carotenoid biosynthetic pathway of tomato, four homozygous and heterozygous SNPs were identified in lycopene epsilon-cyclase gene at the upstream of Exon 1 (1 SNP) and the intronic region between Exons 1 and 2 (3 SNPs) based on multiple sequence alignment of the processing tomato hybrid ‘Ilocos Red’ and table type inbred ‘Hawaii7996’. These SNPs may have a regulatory association with variations in tomato carotenoid metabolism. Interestingly, no sequence difference was found between FLA456 and ‘Super Apollo’ despite being characterized by orange and red fruit colors, respectively. The results support prior studies suggesting that lycopene cyclase genes are transcriptionally controlled as evidenced by their highly conserved sequences. The SNPs characterized in this study at the promoter and intronic regions of lycopene epsilon-cyclase are starting loci to investigate further the genetic control of this gene in regulating carotenoid metabolism and products that result in varying tomato fruit phenotypes.
利用EcoTILLING和测序技术揭示番茄红素epsilon环化酶基因在部分番茄基因型中的变异
选择果实颜色为橙色和红色的番茄基因型,对参与番茄红素环化的候选基因的序列水平变异进行了研究。设计了番茄红素β -环化酶(3)和番茄红素epsilon-环化酶(1)基因序列特异性标记,并通过Ecotype Targeted Induced Local病变IN Genome (EcoTILLING)和Sanger测序筛选推测的单核苷酸多态性(snp)。虽然在番茄类胡萝卜素生物合成途径中被认为是进化保守的基因之一,但通过对加工番茄杂交种Ilocos Red和表型自交系Hawaii7996的多序列比对,在番茄红素epsilon环化酶基因1外显子上游(1 SNP)和1外显子与2外显子之间的内含子区域(3 SNP)鉴定出4个纯合和杂合SNP。这些snp可能与番茄类胡萝卜素代谢的变化具有调节关联。有趣的是,FLA456和“超级阿波罗”之间没有发现序列差异,尽管它们分别具有橙色和红色的水果色。结果支持先前的研究表明,番茄红素环化酶基因是转录控制的,证明了其高度保守的序列。本研究中在番茄红素epsilon环化酶启动子和内含子区域发现的snp是进一步研究该基因在调节类胡萝卜素代谢和导致不同番茄果实表型的产物中的遗传控制的开始位点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Philippine Journal of Science
Philippine Journal of Science Multidisciplinary-Multidisciplinary
CiteScore
1.20
自引率
0.00%
发文量
55
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