Simultaneous Estimation of Hydrochlorothiazide and Losartan Potassium in Bulk Solid Dosage Form by Chemometric Assisted Spectrophotometric Methods

Abstract

A simple UV-visible spectroscopic method was developed and Chemo metric designs were applied for the simultaneous estimation of hydrochlorothiazide (HCT) and losartan potassium (LST) in bulk and solid dosage form. The spectroscopic method was developed by using alcohol as solvent for the two drugs and the data generated from the spectra were mined by using Chemo metric methods such as bi-linear regression analysis, Cramer’s matrix method, Method of least squares, The wavelengths selected for all the above methods were 271 nm (wavelength of maximum absorption; λmax of HCT), 213 nm (wavelength of maximum absorption; λmax of LST). Results: The methods hold good linearity for HCT from 1-5μg/ml, for LST from 2-10μg/ml with regression coefficient values of 0.945 and 0.964 respectively. The intraday and inter-day precision was found to be less than 2% RSD. The percentage recovery and percentage assay was in the range 85-105% of for hydrochlorothiazide (HCT) and losartan potassium (LST) by all the methods. Conclusion: The developed methods neither require any cumbersome separation procedure nor complex derivatization procedures for the analysis of the two drugs and more over they are effective in minimizing the errors in analysis, simple and economical.