Comparison of four semi-quantitative tests for evaluation of colostrum quality in Saanen goats

IF 0.7 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

South African Journal of Animal Science Pub Date : 2022-05-10 DOI:10.4314/sajas.v51i5.12

Y. Kaçar, Z. Mecitoglu, O. Topal, H. Batmaz

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引用次数: 1

Abstract

The aim of this study was to investigate the efficacy of Brix refractometry and to compare the Brix value with semi-quantitative indicators such as total protein (TP), total protein in whey (TPw), glutaraldehyde coagulation time (GCT) and gamma-glutamyl transferase (GGT) enzyme activity for determining the quality of colostrum. Colostrum samples were collected from 38 Saanen goats just after parturition and on the first day (24 ± 4 hours) and second day (48 ± 4) after parturition. The Brix value, TP and TPw levels were measured with optic refractometers. The level of GCT was determined with a 10% glutaraldehyde solution. Immunoglobulin G (IgG) concentrations were measured with a goat IgG ELISA. All measurements decreased significantly after parturition. The IgG and Brix values on the day of parturition were 4719.28 ±107.94 mg/dL and 20.55 ± 0.71, respectively. The TPw levels were lower than TP on all three days and a significant difference was detected on day 2. The IgG concentration was higher in the first-parity and second-parity goats compared with those older does. However, no differences were observed in the other characteristics of the colostrum. Litter size did not affect IgG or the other semi-quantitative tests. Correlation coefficients were higher than 0.8 Tp with TPw, and for the Brix value with both TP and TPw. Brix refractometry could be used to evaluate colostrum quality in Saanen goats and TPw is a more reliable indicator than TP.

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沙嫩山羊初乳质量评价四种半定量试验的比较

本研究旨在探讨白度折光法测定初乳质量的有效性，并将白度值与总蛋白(TP)、乳清总蛋白(TPw)、戊二醛凝固时间(GCT)和γ -谷氨酰转移酶(GGT)酶活性等半定量指标进行比较。选取38只沙嫩山羊，分别于分娩后第1天(24±4小时)和第2天(48±4小时)采集初乳样品。用光学折光仪测定白锐度值、TP和TPw水平。用10%戊二醛溶液测定GCT水平。采用山羊IgG ELISA法检测免疫球蛋白G (IgG)浓度。分娩后各项指标均显著下降。分娩当日IgG和Brix值分别为4719.28±107.94 mg/dL和20.55±0.71 mg/dL。3天TPw水平均低于TP水平，第2天差异显著。第一胎和第二胎山羊IgG浓度高于年龄较大的山羊。然而，在初乳的其他特征上没有观察到差异。产仔数不影响IgG或其他半定量测试。Tp与TPw的相关系数均大于0.8，白锐度值与Tp和TPw的相关系数均大于0.8。白度折光法可用于评价沙嫩山羊初乳质量，TPw比TP更可靠。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

South African Journal of Animal Science 农林科学-奶制品与动物科学

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： The South African Journal of Animal Science is an open access, peer-reviewed journal for publication of original scientific articles and reviews in the field of animal science. The journal publishes reports of research dealing with production of farmed animal species (cattle, sheep, goats, pigs, horses, poultry and ostriches), as well as pertinent aspects of research on aquatic and wildlife species. Disciplines covered nutrition, genetics, physiology, and production systems. Systematic research on animal products, behaviour, and welfare are also invited. Rigorous testing of well-specified hypotheses is expected.