Effects 4-(1-adamantyl)-phenoxy-3-(n-benzyl, n-dimethylamino)-2-propanol chloride on the strains of Pseudomonas Spp.

Abstract

Pseudomonas aeruginosa is one of the main pathogens of nosocomial infections. High resistance of P. aeruginosa to modern antimicrobial agents leads to the decrease in the effectiveness of antibiotic chemotherapy and the need to search for new active compounds. Adamantane derivatives with a wide range of biological activity can be considered as a promising class of substances with an antimicrobial effect. Aim. In the present study, our purpose was to examine susceptibility and ultrastructural alterations of P. aeruginosa cells under the influence of 4-(1-adamantyl)-phenoxy-3-(N-benzyl, N-dimethylamino)-2-propanol chloride (compound KVM-97). Materials and methods. The antimicrobial activity assay of tested compound against bacteria of genus Pseudomonas was determined by serial dilution test in broth. Bacterial cells were exposed to the 0.5 MIC and 2.0 MIC of the KVM-97 for 1, 3, 6 and 24 h. Ultrastructure of intact and treated P. aeruginosa cells was examined by transmission electron microscopy after contrasting by uranyl acetate and lead citrate. Results. It was shown, that compound KVM-97 inhibited Pseudomonas spp growth at concentration 2.5 μg/ml. Examination of P. aeruginosa ultrastructure using electron microscopy showed that the cell and cytoplasmic membrane were damaged in the presence of KVM-97 (invaginations, ruptures) with followed disorganization of cell contents, lysis and cell death. These changes are dose-dependent, they are registered after 1 hour of exposure with the compound and intensified with the time of incubation. Conclusions. A study carried out with KVM-97 has shown that the compound possesses significant inhibitory activity against tested bacterial strains. The detected ultrastructural alterations of P. aeruginosa suggest the possible mechanism of action of 4-(1-adamantyl)-phenoxy-3-(N-benzyl, N-dimethylamino)-2-propanol chloride due to its influence on the membrane apparat in bacterial cell