Radical Scavenging Activities of Albumin-Copper Complex against Bromobenzene Induced Hepatotoxicity

Journal of Clinical Toxicology Pub Date : 2019-01-01 DOI:10.4172/2161-0495.1000405

D. S. Abdel-Magiud, A. Nassar, A. Shatat, A. Mohamed

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引用次数: 1

Abstract

This study was performed to examine that copper-albumin complex can restore oxidative damage and cytotoxicity in induced by Bromobenzene (BB) in liver. Rats were divided into three groups. Group I served as control received corn oil only. Group II exposed to BB at a dose of 300 mg/Kg by weight, twice/week, orally, dissolved in corn oil for one month. Group III received BB as previous dose plus copper albumin- Serum total protein and albumin showed significant reduction while the activities of ALT and AST enzymes significantly increased in BB-treated group when compared to control. The antioxidant enzymes activities were found to be decreased in BB treated rats in contrast nitric oxide and lipid peroxidation levels were increased in comparison with normal control. Histopathology revealed that BB had a hepatotoxic effects represented by hepatitis and fatty degeneration. Nile blue positive stain showed lipofuscin and/or ceroid granules. Necrotic nuclei with disintegrated DNA stained with acridine orange. The treated rats with copper-albumin complex reversed the most of parameters to normal control.

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白蛋白-铜复合物对溴苯所致肝毒性的清除活性

本研究旨在探讨铜-白蛋白复合物对溴苯(BB)所致肝脏氧化损伤及细胞毒性的恢复作用。大鼠被分为三组。第一组为对照组，仅饲喂玉米油。II组以300 mg/Kg(按重量计)的剂量，每周2次，口服，溶解于玉米油中，持续1个月。III组以BB为原剂量加铜白蛋白治疗，与对照组相比，BB治疗组血清总蛋白和白蛋白显著降低，ALT和AST活性显著升高。与正常对照相比，BB处理大鼠抗氧化酶活性降低，一氧化氮和脂质过氧化水平升高。组织病理学显示BB具有肝毒性作用，表现为肝炎和脂肪变性。尼罗蓝阳性染色显示脂褐质和/或蜡样颗粒。细胞核坏死，DNA解体，染色为吖啶橙。用铜-白蛋白复合物治疗的大鼠大部分参数与正常对照相反。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of Clinical Toxicology

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