Evaluation of chemical profile and antioxidant potential of Trichodesma indicum (L.) R.Br.

International Journal of Phytomedicine Pub Date : 2017-09-02 DOI:10.5138/09750185.2012

Mahendra S. Khyade, Subhash N Varpe, Anup D. Padwal

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引用次数: 6

Abstract

The present study was conducted to evaluate the antioxidant activity and chemical composition of aqueous methanolic extract of Trichodesma indicum (L.) R.Br. The extract was tested for antioxidant activity using various in vitro models viz., DPPH, ABTS, NO, FRAP, total antioxidant activity and reducing power. The total phenolic and flavonoid contents were found to be equivalents to 97.83 ± 0.08 µg of gallic acid and 45.45 ± 0.19 µg of rutin /mg of dried aqueous methanolic extract, respectively. Among various antioxidant assays performed, maximum inhibition was observed in the ABTS (IC 50 17.13 ± 0.23 μg) followed by DPPH (IC 50 80.64 ± 0.29 μg) and NO (IC 50 4.12 ± 0.02 μg) assay. GC-MS analysis revealed the presence of over 38 compounds, the prevailing compounds were hexadecanoic acid, β-sitosterol, ethyl iso-allocholate and 9,12,15,-Octadecatrienoicacid. The HPLC analysis further confirmed the presence of rutin, salicylic acid and benzoic acid. This confirms that T. indicum could be the good source of natural antioxidant for industrial and pharmaceutical preparations.

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indicum (L.)木霉(Trichodesma indicum)的化学特性及抗氧化潜力评价R.Br。

本文研究了木霉(Trichodesma indicum, L.)乙醇水提物的抗氧化活性及其化学成分。R.Br。采用DPPH、ABTS、NO、FRAP、总抗氧化活性和还原力等体外模型检测提取物的抗氧化活性。总酚和类黄酮含量分别相当于没食子酸(97.83±0.08µg)和芦丁(45.45±0.19µg) /mg。在抗氧化实验中，ABTS (ic50为17.13±0.23 μg)的抑制作用最大，其次是DPPH (ic50为80.64±0.29 μg)和NO (ic50为4.12±0.02 μg)。气相色谱-质谱分析显示其含有38种化合物，主要化合物为十六烷酸、β-谷甾醇、异胆酸乙酯和9,12,15，-十八碳三烯酸。HPLC分析进一步证实了芦丁、水杨酸和苯甲酸的存在。这证实了稻瘟病菌可作为天然抗氧化剂的良好来源用于工业和药物制剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Journal of Phytomedicine

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