Evolution of the complex treatment of purulent diseases of the hand including laser irradiation (a review)

Abstract

Effects of 1270 nm laser light irradiation at phage particles of virulent klebsiellosis bacteriophage were studied. The medical klebsiellosis bacteriophage, manufactured industrially, was taken as the study object. Klebsiella pneumonia N 296, sensitive to the selected phage, was used as a test-culture. An experimental device manufactured by LTD «New surgical technologies» was used as a source of light. Semiconductor diodes generate light with wavelength 1270 nm (1268–1272 nm) in the continuous mode. The number of viable phage particles in the initial solution of klebsiellosis bacteriophage was 5×108. Irradiation of the phage with 1270 nm laser light decreased the number of viable phage particles to 105. Results did not practically depend on the exposure time, i. e. phage titers were equally reduced when exposed to laser light for 5 min, 10 min and 15 min. Irradiation of Klebsiella bacteriophage with 1270 nm laser light reduced the number of viable phage particles by 3 log orders (initial titer was 108; after irradiation – 105 negative phage colonies). It is indicative of their damage. Mechanisms of phage particle damage should be the object of further research so as to defi ne if laser irradiation with the above mentioned wavelengths can be used in medical practice.