Influence of Additional Nutrients and Gelling Agents on in Vitro Response of Selected Indica Rice Varieties

Abstract

Indica rice varieties are recalcitrant to culture and hence the culture media should be supplemented with additional nutrients to provide energy and osmotic potential for best in vitro response. Combinations of plant growth regulators have profound influence on callus induction and regeneration potential of the selected genotypes. In addition, concentration and choice of gelling agents also have their effect on regeneration of indica rice varieties. Impact of L-Proline, and Casein Hydrolysate on tissue culture response of selected indica rice varieties is discussed and the best choice of gelling agent and their in vitro response is elucidated.