Molecular Typing of Uropathogenic Escherichia coli Strains Isolated from Patients in Gorgan by Random Amplified Polymorphic DNA-PCR (RAPD-PCR)

Medical laboratory sciences Pub Date : 2021-10-18 DOI:10.22037/AMLS.V7.34383

Zeynab Faraji, Behnoush Khasheii, E. Ghaemi, Shaghayegh Anvari, R. Rajabnia, A. Jamali

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Abstract

Background and Aim: Urinary tract infections (UTIs) are one of the most common pathological diseases in communities and hospitals, often caused by uropathogenic Escherichia coli (UPEC). The use of microbial strains typing is an integral part of epidemiological surveys of infectious diseases to identify epidemics, detect the infection source, track and recognize pathogenic strains. In this study, uropathogenic Escherichia coli (UPEC) strains isolated from patients living in Gorgan were typed using RAPD-PCR. Methods: In total, 187 Escherichia coli strains isolated from urine samples of inpatients and outpatients of Gorgan city from 2010 to 2016 were analyzed by the RAPD-PCR method using two primers. Using GelClust and FigTree software, the respective dendrograms were plotted by unweighted pair group method with arithmetic mean (UPGMA). Results: In our research, 614 bands were detected using two primers. The highest frequency of bands was obtained in 400 bp and 500 bp with 65 repeats and the lowest number of bands was in 2500 bp and 3000 bp with one repeat and 32 clusters. The largest number of isolates (i.e.14) was placed in cluster 16. Most bands were polymorphic, indicating high genetic diversity in isolates. Conclusion: Analysis of 32 clusters of our study by the RAPD-PCR method showed that the studied clusters do not have a specific and unique feature and the scattering of isolates properties are equal among the clusters. Because each cluster had its characteristics, E. coli strains in the region have great genetic diversity. *Corresponding Author: Ailar Jamali; Email: jamali@goums.ac.ir; ORCID: https://orcid.org/0000-0002-4612-8144 Please cite this article as: Faraji Z, Khasheii B, Ghaemi EA, Anvari S, Rajabnia R, Jamali A. Molecular Typing of Uropathogenic Escherichia coli Strains Isolated from Patients in Gorgan by Random Amplified Polymorphic DNA-PCR (RAPD-PCR). Arch Med Lab Sci. 2021;7:1-7 (e17). https://doi.org/10.22037/amls.v7.34383

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随机扩增多态性DNA-PCR (RAPD-PCR)对泌尿生殖腺患者尿路致病性大肠杆菌分子分型的研究

背景与目的:尿路感染(UTIs)是社区和医院最常见的病理疾病之一，多由尿路致病性大肠杆菌(UPEC)引起。微生物菌株分型是传染病流行病学调查的重要组成部分，用于确定流行病、检测感染源、跟踪和识别致病菌株。本研究采用RAPD-PCR方法对从戈尔根患者中分离的尿路致病性大肠杆菌(UPEC)进行分型。方法:采用两种引物，采用RAPD-PCR方法对2010 - 2016年戈尔根市住院和门诊患者尿液中分离的187株大肠埃希菌进行分析。利用GelClust和FigTree软件，采用带算术平均值的非加权对群法(UPGMA)绘制各自的树形图。结果:两种引物共检测到614条条带。条带频率最高的是400 bp和500 bp，重复65次;条带数量最少的是2500 bp和3000 bp，重复1次，32个簇。最大数量的分离株(即14株)位于聚类16。大多数条带具有多态性，表明分离物具有较高的遗传多样性。结论:采用RAPD-PCR方法对本研究的32个聚类进行分析，发现所研究的聚类没有特定的、独特的特征，分离物的分散特性在聚类之间是相等的。由于每个集群都有自己的特点，该地区的大肠杆菌菌株具有很大的遗传多样性。*通讯作者:Ailar Jamali;电子邮件:jamali@goums.ac.ir;Faraji Z, Khasheii B, Ghaemi EA, Anvari S, Rajabnia R, Jamali A.用随机扩增多态性DNA-PCR (RAPD-PCR)对泌尿生殖腺患者尿路致病性大肠杆菌进行分子分型。中华医学杂志，2011;7:1-7 (e17)。https://doi.org/10.22037/amls.v7.34383

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Medical laboratory sciences

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