Altered odor-induced expression of c-fos and arg 3.1 immediate early genes in the olfactory system after familiarization with an odor.

M. Montag-Sallaz, N. Buonviso
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引用次数: 36

Abstract

In adult rats, repeated exposure to an odorant, in absence of any experimentally delivered reinforcement, leads to a drastic decrease in mitral/tufted (M/T) cell responsiveness, not only for the familiar odor but also for other novel odors. In the present study, using two different and complementary in situ hybridization methods, we analyzed the effect of familiarization with an odorant on c-fos and arg 3.1 mRNA expression levels, and we examined the odor specificity of this effect. Odor exposure induces a specific increase in c-fos and arg 3.1 expression in some particular olfactory bulb quadrants. Previous familiarization with the test odor results in a decreased expression of both IEGs in these quadrants, leading to the alteration of the odor-specific pattern of c-fos and arg 3.1 expression. In contrast, this odor-specific pattern is not affected when different odors are used for familiarization and test. Similarly, an odor-specific familiarization effect leading to a reduced c-fos and arg 3.1 expression was also detected in the cingulate cortex and in the anterior piriform cortex. These results support our hypothesis that the decrease in M/T cell responsiveness following a preceding familiarization with an odorant may be related to a particular form of synaptic plasticity involving changes at the genomic level, and reveals further insight in olfactory information processing and the cellular mechanisms underlying familiarization in the olfactory system.
熟悉气味后嗅觉系统中c-fos和arg 3.1即时早期基因表达的改变
在成年大鼠中,在没有任何实验传递强化的情况下,反复暴露于气味剂会导致二尖瓣/簇状细胞(M/T)反应性急剧下降,不仅对熟悉的气味,而且对其他新气味也是如此。在本研究中,我们采用两种不同的互补原位杂交方法,分析了熟悉气味剂对c-fos和arg 3.1 mRNA表达水平的影响,并检验了这种影响的气味特异性。气味暴露诱导某些特定嗅球象限中c-fos和arg 3.1表达的特异性增加。先前对测试气味的熟悉导致这些象限中两种eeg的表达减少,导致c-fos和arg 3.1的气味特异性表达模式发生改变。相反,当不同的气味被用于熟悉和测试时,这种特定气味的模式不受影响。同样,在扣带皮层和前梨状皮质中也检测到导致c-fos和arg 3.1表达减少的气味特异性熟悉效应。这些结果支持了我们的假设,即在先前熟悉气味后,M/T细胞反应性的降低可能与涉及基因组水平变化的特定形式的突触可塑性有关,并进一步揭示了嗅觉信息处理和嗅觉系统中熟悉的细胞机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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