Ferric oxide nanoparticles administration suppresses isoniazid induced oxidative stress in the rat brain tissue

Abstract

Isoniazid is one of the anti-tuberculosis therapeutic agents capable of causing side effects such as oxi dative stress, brain tissue damage and mental disorders. This study aimed to investigate the effect of ferric oxide (Fe 2 o 3 ) nanoparticles administration on isoniazid-induced oxidative stress parameters in rat brain tissue. Forty adult male Wistar rats (200–250 g) were randomly divided into a group with no treatment as control and four experimental groups. Animals of experimental groups received intraperitoneally for 12 days daily saline, 50 mg/kg of isoniazid, 50 mg/kg of isoniazid and 0.2 or 0.4 mg/kg Fe 2 o 3 nanoparticles accordingly. The activity of catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), the level of glutathione (gSH), malondialdehyde (mda) and total protein were determined in brain tissue homogenates by spectrophotometric methods. It was shown that Cat and gSt activities, as well as gSH and mda levels in the brain tissue of animals in the isoniazid-treated group were increased compared with the control untreated group, while following the treatment with 0.2 or 0.4 mg/kg Fe 2 o 3 nanoparticles the studied oxidative stress parameters returned to the control level (P < 0.05). No changes in Sod activity in any of the treated groups were observed compared to the control. This study showed that the administration of ferric oxide nanoparti cles can suppress isoniazid-induced oxidative stress in the brain tissue of rats mentally damaged by isoniazid.