Human Rhinovirus Molecular Epidemiology and Genotyping in Iranian Military Trainees with Acute Respiratory Symptoms

F. S. Tameshkel, Ali Salimi Jeda, A. Tavakoli, M. K. Niya, M. Izadi, F. Zamani, H. Keyvani
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Abstract

Background: Human rhinovirus (HRV) is still the most prevalent viral infection in humans and a significant cause of acute respiratory tract infections (ARTIs) in many communities, including military personnel undergoing basic training. Objectives: In this research, we assessed the molecular epidemiology, genotyping, and phylogenetic classification of HRVs in Iranian military trainees with respiratory infections (RI). Methods: For HRV identification and genotyping, respiratory specimens were obtained, and RT-PCR was conducted for genotyping and phylogenetic analysis of HRV utilizing primers for the 5-UTR region. Results: Among 400 Iranian military trainees (average age of 21 ± 4 years, the range of 18 - 57 years) with respiratory infections, HRV was detected in 29 patients (7%) using RT-PCR. The direct sequencing of PCR products from 10 specimens showed that the incidence of type A (n = 5, 50%) was higher than that of type B (n = 4, 40%) and type C (n = 1, 10%). There were no significant associations between HRV and respiratory and clinical symptoms, blood group, and indoor or outdoor conditions (P-value > 0.05). Conclusions: This research was the first to record HRV as a significant cause of respiratory problems among military trainees in Iran, with a frequency of 7%. The most prevalent genotype was HRV-A, which may be applicable in epidemiological and clinical studies, as well as vaccination plans.
伊朗军事学员急性呼吸道症状的人鼻病毒分子流行病学和基因分型
背景:人鼻病毒(HRV)仍然是人类中最流行的病毒感染,也是许多社区(包括接受基础训练的军事人员)急性呼吸道感染(ARTIs)的重要原因。目的:在本研究中,我们评估了伊朗军事学员呼吸道感染(RI)的hrv的分子流行病学、基因分型和系统发育分类。方法:采集呼吸道标本进行HRV鉴定和基因分型,利用5-UTR区引物进行RT-PCR分型和系统发育分析。结果:400例伊朗军事学员(平均年龄21±4岁,18 ~ 57岁)呼吸道感染患者中,RT-PCR检测出HRV 29例(7%)。对10份标本的PCR产物直接测序结果显示,A型(n = 5, 50%)的发病率高于B型(n = 4, 40%)和C型(n = 1, 10%)。HRV与呼吸症状、临床症状、血型、室内外条件无显著相关性(p值> 0.05)。结论:这项研究首次记录了HRV是伊朗军事受训人员呼吸问题的重要原因,发病率为7%。最普遍的基因型是HRV-A,这可能适用于流行病学和临床研究,以及疫苗接种计划。
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