Efficacy of Butanolic Fraction of Cocos Nucifera's Root Aqueous Extract on Induced Anemia Treatment

Abstract

Cocos nucifera was a coastal plant whose roots were used in pharmacopoeia to treat anemia in Benin. The aqueous extract from its roots stimulated the synthesis of hemoglobin. The aim of this work was to test in vivo the efficacy of the butanolic fraction of the extract in the treatment of anemia. Methods: Wistar rats were anemic with phenylhydrazine for two days. From D2 to D15, some were treated by gavage with the butanolic fraction of the aqueous extract of Cocos nucifera roots at the dose of 40 mg or 60 mg/kg of body weight/day, others were treated with vitafer (an anti-anemic drug) or with distilled water. The rats blood were collected on days D0, D2, D7, D10 and D15 for the complete blood count and the osmotic resistance of the red blood cells. Results: On D2, phenylhydrazine significantly lowered the hemoglobin level and the number of red blood cells, which were respectively corrected on D10 and D15 by the fraction of extract with release of hypochromic macrocytes. However, the effect was slower than that of the crude extract, was not specific to erythropoiesis because it also stimulated thrombopoiesis and was not dose-dependent. Conclusion: The butanolic fraction of the aqueous extract of Cocos nucifera roots corrected anemia by stimulation of hematopoiesis. The observed biological activity would probably be linked to anthocyanins which are mainly isolated by butanol. These results contribute to a better knowledge of bioactive compounds of our antianemic plants.