Effects of iodine excess on mitochondrial superoxide production and mitochondrial membrane potential in rat thyroid cell line cells

Q3 Medicine

中华地方病学杂志 Pub Date : 2010-05-20 DOI:10.3760/CMA.J.ISSN.1000-4955.2010.03.003

L. Min, Yao Xiao-mei, Chen Zu-pei, Liu Lan-ying

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引用次数: 0

Abstract

Objective To investigate the effects of iodine excess on mitochondrial superoxide production and mitoehondrial membrane potential(△ψ)changes in Fisher rat thyroid cell line(FRTL)cells.Methods FRTL cells were treated with 10-4mol/L potassium iodine(KI),10 U/L thyrotropin(TSH),10-4 mol/L KI+10 U/L TSH respectively for 24 h.Effects on cell proliferation were assayed by methyl thiazolyl tetrazolium(MTT)colorimetric method.Changes of mitochondrial superoxide production and △ψ were measured by live cell imaging and spectrofluorometer using MitoSOX and rhodamine 123(rh123)respectively.Results Absorbance(A)in the KI group (0.794±0.144)showed a significant decline compared to the control group(1.000 ±0.183,P 0.05),but the former was marked higher than the KI group(P 0.05). Conclusion Iodine excess (10-4 mol/L KI) may lead to peroxide damage on the mitochondria of FRTL cells, and cell proliferation is inhibited. Combining treatment with 10 U/L TSH may attenuate mitochondrial peroxide damage and inhibition of cell proliferation caused by iodine excess. Key words: Iodine; Thyroid gland; Superoxides; Membrane potential, mitochondrial; Thyrotropin

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碘过量对大鼠甲状腺细胞系细胞线粒体超氧化物生成和线粒体膜电位的影响

目的探讨碘过量对Fisher大鼠甲状腺细胞系(FRTL)线粒体超氧化物生成及线粒体膜电位(△ψ)变化的影响。方法分别用10-4mol/L碘化钾(KI)、10 U/L促甲状腺素(TSH)、10-4mol/L碘化钾+10 U/L TSH处理FRTL细胞24 h，采用甲基噻唑四氮唑(MTT)比色法检测对细胞增殖的影响。采用MitoSOX和rhodamine 123(rh123)分别用活细胞成像和荧光光谱仪测量线粒体超氧化物产量和△ψ的变化。结果KI组吸光度(A)(0.794±0.144)明显低于对照组(1.000±0.183)(P 0.05)，但明显高于KI组(P 0.05)。结论碘过量(10-4 mol/L KI)可引起FRTL细胞线粒体过氧化损伤，抑制细胞增殖。10 U/L TSH联合处理可减轻碘过量引起的线粒体过氧化损伤和细胞增殖抑制。关键词:碘;甲状腺;过氧化物;膜电位，线粒体;促甲状腺素

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来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.