Flexible microfluidic bio-lab-on-a-chip multi-sensor platform for electrochemical measurements

IEEE SENSORS 2014 Proceedings Pub Date : 2014-12-15 DOI:10.1109/ICSENS.2014.6985176

A. Moya, X. Illa, E. Prats-Alfonso, N. Zine, G. Gabriel, A. Errachid, R. Villa

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引用次数: 1

Abstract

A novel polymeric microfluidic multi-sensor system has been developed using rapid prototyping techniques and low-cost materials which permits the dynamic online monitoring of several cell culture parameters in a wide range of biomedical applications. The multi-sensor is made of polymeric flexible substrate with integrated gold microband electrodes for electrochemical measurements of Dissolved Oxygen (DO) and Hydrogen (H+), Sodium (Na+) and Potassium (K+) cations. The platform integrates several working electrodes (WE), pseudoreferences (pRE) and a counter electrode (CE) for electrochemical detection. The bio-lab-on-a-chip allows the detection of changes in real time, with rapid response, allowing to measure small volumes and fast cell metabolisms changes. The reliable analytical performance of the platform in in-vitro conditions has been demonstrated. DO was detected with activated gold electrode, and H+, Na+ and K+ were detected with the developed ion-selective microelectrodes (μISE) using electropolymerized polypyrrole (PPy) film as a contact layer, allowing a good performance of the sensor.

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用于电化学测量的柔性微流控生物芯片实验室多传感器平台

利用快速成型技术和低成本材料开发了一种新型聚合物微流控多传感器系统，该系统可以在广泛的生物医学应用中动态在线监测几个细胞培养参数。该多传感器由聚合物柔性衬底和集成金微带电极制成，用于溶解氧(DO)和氢(H+)、钠(Na+)和钾(K+)阳离子的电化学测量。该平台集成了几个工作电极(WE)、假参考电极(pRE)和一个对电极(CE)，用于电化学检测。生物实验室芯片允许实时检测变化，具有快速响应，允许测量小体积和快速细胞代谢变化。该平台在体外条件下具有可靠的分析性能。采用活化金电极检测DO，采用电聚合聚吡咯(PPy)薄膜作为接触层的离子选择性微电极(μISE)检测H+、Na+和K+，使传感器具有良好的性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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IEEE SENSORS 2014 Proceedings

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