New Approach For Simvastatin As An Antibacterial: Synergistic Effect With Bio-Synthesized Silver Nanoparticles Against Multidrug-Resistant Bacteria.

IF 0.8 Q4 Environmental Science

Houille Blanche-Revue Internationale De L Eau Pub Date : 2019-10-03 eCollection Date: 2019-01-01 DOI:10.2147/IJN.S211756

E P Figueiredo, J M Ribeiro, E K Nishio, S Scandorieiro, A F Costa, V F Cardozo, A G Oliveira, N Durán, L A Panagio, Rkt Kobayashi, G Nakazato

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引用次数: 0

Abstract

Background: Multidrug-resistant bacteria such as extended-spectrum beta-lactamase (ESBL), Enterobacteriaceae, and methicillin-resistant Staphylococcus aureus (MRSA) pose a challenge to the human health care system. MRSA is among the major causes of hospital-acquired and community infections.

Methods: Therefore, in the present study, we evaluated the antibacterial activity of silver nanoparticles synthesized by Fusarium oxysporum (AgNP_bio) in combination with simvastatin against reference and multidrug-resistant bacterial strains.

Results: Simvastatin showed a minimal inhibitory concentration (MIC) ranging from 0.062 to 0.25 mg mL^-1 against MRSA. AgNP_bio with a size of 77.68± 33.95 nm and zeta potential -34.6 ± 12.7 mV showed an MIC of 0.212 mg mL^-1 against S. aureus including MRSA strains. The checkerboard assay and time-kill curves exhibited a synergistic effect of the simvastatin-AgNP_bio combination on antibacterial activity against MRSA strains. The combination of simvastatin and AgNP_bio demonstrated antibacterial activity against Escherichia coli producing ESBL. Scanning electron microscopy showed the formation of cell surface protrusions after treatment with AgNP_bio and the formation of a large amorphous mass after treatment with simvastatin, both in MRSA.

Conclusion: Our results indicate that the combination of AgNP_bio and simvastatin could be a great future alternative in the control of bacterial infections, where, when combined with simvastatin, smaller doses of AgNP_bio are required, with the same antibacterial activity.

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辛伐他汀抗菌新方法：辛伐他汀作为抗菌剂的新方法：与生物合成的银纳米粒子对耐多药细菌的协同效应

背景：耐多药细菌（如广谱β-内酰胺酶（ESBL）、肠杆菌科细菌和耐甲氧西林金黄色葡萄球菌（MRSA））对人类医疗保健系统构成了挑战。MRSA 是医院感染和社区感染的主要原因之一：因此，在本研究中，我们评估了由氧孢镰刀菌合成的银纳米粒子（AgNPbio）与辛伐他汀联用对参考菌株和多重耐药菌株的抗菌活性：辛伐他汀对 MRSA 的最小抑菌浓度（MIC）为 0.062 至 0.25 毫克毫升/升。AgNPbio 大小为 77.68± 33.95 nm，Zeta 电位为 -34.6 ± 12.7 mV，对金黄色葡萄球菌（包括 MRSA 菌株）的 MIC 为 0.212 mg mL-1。棋盘试验和时间杀伤曲线显示，辛伐他汀-AgNPbio 组合对 MRSA 菌株的抗菌活性具有协同效应。辛伐他汀和 AgNPbio 的组合对产生 ESBL 的大肠杆菌具有抗菌活性。扫描电子显微镜显示，在使用 AgNPbio 处理 MRSA 菌株后，细胞表面突起形成，而在使用辛伐他汀处理 MRSA 菌株后，细胞表面大块无定形物质形成：我们的研究结果表明，AgNPbio 与辛伐他汀的结合可能是未来控制细菌感染的一个重要选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Houille Blanche-Revue Internationale De L Eau 环境科学-水资源

CiteScore

0.40

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： La Houille Blanche, revue internationale de l ''eau/International Water Journal, is the only almost all French-written journal of Water science. It promotes the dissemination of research and innovative practices in the field of water, as a key resource for everyday use for human needs, agriculture, energy and transport, and hydraulic public works. This includes environmental and risk assessment and management issues related to hydrology, meteorology, flood, low flows and drought, as well as issues in the field of fluid mechanics, hydraulics machinery, multiphase flows, microfluidics... La Houille Blanche, International Water Journal, is cited by Science Citation Index Expanded of Institute for Scientific Information (I.S.I) in USA, and by CNRS in France; since 1902, the journal publishes high-standard relevant peer-reviewed research papers. La Houille Blanche also provides more general information and recent news about the water world, and the SHF association and its members life: scientific events, book review, R & D advancements, … Six issues per year. Thematical journal for original articles and reviews mostly in French.