{"title":"Hexavalent chromium reduction and 16S rDNA identification of bacteria isolated from a Cr (VI) contaminated site","authors":"A. Das, Susmita Mishra","doi":"10.5580/10ae","DOIUrl":null,"url":null,"abstract":"A Gram-positive, hexavalent chromium [chromate: Cr (VI)]-resistant & reducing bacterium, isolated from sukind chromite mines, jajpur, India, was identified as a Brevibacterium casei(Gene Bank Accession Number: EU781952) by gene sequence homology. The strain(designated as APD15) could tolerate chromium up to a maximum concentration of 500 ppm, at optimum temperature and pH 30C and 7 for maximum chromium reduction. Agar supplemented with 100g Cr (VI)/ml as K2Cr2O7 and 0.5% (w/v) dextrose used as a carbon source. The results of the study indicated removal of more than 94% chromium (VI) by Brevibacterium caseidetermined by diphenylcarbazide colorimetric assay.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Internet journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5580/10ae","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 7
Abstract
A Gram-positive, hexavalent chromium [chromate: Cr (VI)]-resistant & reducing bacterium, isolated from sukind chromite mines, jajpur, India, was identified as a Brevibacterium casei(Gene Bank Accession Number: EU781952) by gene sequence homology. The strain(designated as APD15) could tolerate chromium up to a maximum concentration of 500 ppm, at optimum temperature and pH 30C and 7 for maximum chromium reduction. Agar supplemented with 100g Cr (VI)/ml as K2Cr2O7 and 0.5% (w/v) dextrose used as a carbon source. The results of the study indicated removal of more than 94% chromium (VI) by Brevibacterium caseidetermined by diphenylcarbazide colorimetric assay.