Über den mechanismus der katalase-wasserstoffperoxid-reaktion I. Der disulfid- und sulfhydrylgehalt der rinderleber-katalase

H Hermel, R Havemann
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引用次数: 3

Abstract

  • 1.

    1. The amperometrically titratable disulfide and sulfhydryl content of beef-liver catalase is not constant. It changes with enzyme activity, pH and after the action of weak oxidizing agents. As a rule, the ratio disulfide: sulfhydryl increases with increasing enzyme activity. With variation of pH latent sulfhydryl groups are released, and free sulfhydryl groups are masked. The pK of this equilibrium reaction is 7.18. Through the action of oxidizing agents, some sulfhydryl groups can be oxidized to disulfide groups.

  • 2.

    2. When the sulfhydryl groups of catalase are blocked by Hg2+ or C6H5Hg+ a loss of enzyme activity occurs. The loss of activity with increasing Hg2+ or C6H5Hg+ concentration may be represented as an equilibrium curve. The change of activity on blocking the prosthetic groups with azide can be expressed in the same way.

  • 3.

    3. The dissociation of the proton from the sulfhydryl group was followed by electrometric titration. The dissociation constant was found to be 2.00 · 10−9 M (at 0°).

turner和肿瘤中缺失的活性氢反应机制
1.1. 牛肝过氧化氢酶的可安培滴定二硫和巯基含量不是恒定的。它随酶活性、pH值及弱氧化剂作用而变化。通常,二硫:巯基的比值随着酶活性的增加而增加。随着pH的变化,潜在的巯基被释放,游离的巯基被掩盖。这个平衡反应的pK是7.18。通过氧化剂的作用,一些巯基可以被氧化成二硫基。当过氧化氢酶的巯基被Hg2+或C6H5Hg+阻断时,酶活性就会丧失。随着Hg2+或C6H5Hg+浓度的增加,活性的损失可以用平衡曲线表示。叠氮化物阻断假基后活性的变化也可以用同样的方式表达。质子与巯基的解离用电滴定法进行。解离常数为2.00·10−9 M(0°)。
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