Effect of fluoride on proliferation, differentiation and mRNA expression of osteoprotegerin and receptor activator of nuclear factor κβ ligand in mouse osteoblasts

Q3 Medicine

中华地方病学杂志 Pub Date : 2011-05-20 DOI:10.3760/CMA.J.ISSN.1000-4955.2011.03.003

Guo Xiao-ying, Cai RuoXin, Sun Gui-fan

{"title":"Effect of fluoride on proliferation, differentiation and mRNA expression of osteoprotegerin and receptor activator of nuclear factor κβ ligand in mouse osteoblasts","authors":"Guo Xiao-ying, Cai RuoXin, Sun Gui-fan","doi":"10.3760/CMA.J.ISSN.1000-4955.2011.03.003","DOIUrl":null,"url":null,"abstract":"Objective To investigate the effect of sodium fluoride(NaF) on proliferation, differentiation and the mRNA expression of osteoprotegerin (OPG) and receptor activator of nuclear factor κβ ligand (RAN KL) of mouse osteoblasts. Methods Osteoblasts were isolated from calvarias of Kunming mice born in 1 - 2 d and cultured. Various concentrations of NaF(0, 10-8, 10-7, 10-6, 10-5, 10-4, 10-3mol/L) were added to the culture medium, the proliferation and activity of alkaline phosphatase(ALP) was determined after 72 h or 120 h. The expression of OPG mRNA and RANKL mRNA was analyzed by semi-quantification RT-PCR. Difference among groups was analyzed by One-Way AN0VA. Difference between two groups was analyzed by LSD-t test. Results There was significant difference in cell proliferation among groups after 72 h(F = 13.806, P 0.05). The ratio of RANKL/OPG decreased with increasing doses of fluoride and increased in 10-4, 10-3 mol/L groups, but there was no difference between groups(F = 1.376, P> 0.05). Conclusions A biphasic pattern of proliferation and differentiation has been induced in mouse osteoblasts, which manifests stimulation effect in low doses and suppression in higher doses. Low doses of sodium fluoride suppress differentiation and maturation of osteoblasts by increasing expression of OPG mRNA, while high doses of sodium fluoride enhance differentiation and maturation of osteoblasts by decreasing expression of OPG mRNA. \n \nKey words: \nFluoride; Osteoblasts; Osteoprotegerin; Receptor activator of nuclear factor κβ ligand","PeriodicalId":55880,"journal":{"name":"中华地方病学杂志","volume":"159 1","pages":"243-246"},"PeriodicalIF":0.0000,"publicationDate":"2011-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华地方病学杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1000-4955.2011.03.003","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

Objective To investigate the effect of sodium fluoride(NaF) on proliferation, differentiation and the mRNA expression of osteoprotegerin (OPG) and receptor activator of nuclear factor κβ ligand (RAN KL) of mouse osteoblasts. Methods Osteoblasts were isolated from calvarias of Kunming mice born in 1 - 2 d and cultured. Various concentrations of NaF(0, 10-8, 10-7, 10-6, 10-5, 10-4, 10-3mol/L) were added to the culture medium, the proliferation and activity of alkaline phosphatase(ALP) was determined after 72 h or 120 h. The expression of OPG mRNA and RANKL mRNA was analyzed by semi-quantification RT-PCR. Difference among groups was analyzed by One-Way AN0VA. Difference between two groups was analyzed by LSD-t test. Results There was significant difference in cell proliferation among groups after 72 h(F = 13.806, P 0.05). The ratio of RANKL/OPG decreased with increasing doses of fluoride and increased in 10-4, 10-3 mol/L groups, but there was no difference between groups(F = 1.376, P> 0.05). Conclusions A biphasic pattern of proliferation and differentiation has been induced in mouse osteoblasts, which manifests stimulation effect in low doses and suppression in higher doses. Low doses of sodium fluoride suppress differentiation and maturation of osteoblasts by increasing expression of OPG mRNA, while high doses of sodium fluoride enhance differentiation and maturation of osteoblasts by decreasing expression of OPG mRNA. Key words: Fluoride; Osteoblasts; Osteoprotegerin; Receptor activator of nuclear factor κβ ligand

查看原文本刊更多论文

氟对小鼠成骨细胞增殖、分化及骨保护素和核因子κβ受体激活剂mRNA表达的影响

目的探讨氟化钠(NaF)对小鼠成骨细胞增殖、分化及骨保护素(OPG)和核因子κβ受体激活剂配体(RAN KL) mRNA表达的影响。方法从昆明小鼠1 ~ 2 d颅骨中分离成骨细胞进行培养。在培养液中加入不同浓度的NaF(0、10-8、10-7、10-6、10-5、10-4、10-3mol/L)，分别在72 h和120 h后检测碱性磷酸酶(ALP)的增殖和活性，采用半定量RT-PCR分析OPG mRNA和RANKL mRNA的表达。各组间差异采用单因素方差分析。采用LSD-t检验分析两组间差异。结果72h后各组细胞增殖差异有统计学意义(F = 13.806, p0.05)。RANKL/OPG比值随氟剂量的增加而降低，10-4、10-3 mol/L组升高，但组间差异无统计学意义(F = 1.376, P < 0.05)。结论小鼠成骨细胞具有低剂量刺激、高剂量抑制的增殖分化双期模式。低剂量氟化钠通过增加OPG mRNA的表达抑制成骨细胞的分化和成熟，高剂量氟化钠通过降低OPG mRNA的表达促进成骨细胞的分化和成熟。关键词:氟化物;成骨细胞;Osteoprotegerin;核因子κβ配体受体激活剂

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.