Genome wide conditional mouse knockout resources

Q3 Pharmacology, Toxicology and Pharmaceutics

Drug Discovery Today: Disease Models Pub Date : 2016-06-01 DOI:10.1016/j.ddmod.2017.08.002

C. Kaloff , K. Anastassiadis , A. Ayadi , R. Baldock , J. Beig , M.-C. Birling , A. Bradley , S.D.M. Brown , A. Bürger , W. Bushell , F. Chiani , F.S. Collins , B. Doe , J.T. Eppig , R.H. Finnell , C. Fletcher , P. Flicek , M. Fray , R.H. Friedel , A. Gambadoro , W. Wurst

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引用次数: 5

Abstract

The International Knockout Mouse Consortium (IKMC) developed high throughput gene trapping and gene targeting pipelines that produced mostly conditional mutations of more than 18,500 genes in C57BL/6N mouse embryonic stem (ES) cells which have been archived and are freely available to the research community as a frozen resource. From this unprecedented resource more than 6000 mutant mouse strains have been generated by the IKMC in collaboration with the International Mouse Phenotyping Consortium (IMPC). In addition, a cre-driver resource was established including 250 C57BL/6 cre-inducible mouse strains. Complementing the cre-driver resource, a collection comprising 27 rAAVs expressing cre in a tissue-specific manner has also been produced. All resources are easily accessible from the IKMC/IMPC web portal (www.mousephenotype.org). The IKMC/IMPC resource is a standardized reference library of mouse models with defined genetic backgrounds enabling the analysis of gene-disease associations in mice of different genetic makeup and should therefore have a major impact on biomedical research.

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全基因组条件小鼠基因敲除资源

国际敲除小鼠联盟(IKMC)开发了高通量基因捕获和基因靶向管道，在C57BL/6N小鼠胚胎干(ES)细胞中产生了18,500多个基因的条件突变，这些基因已存档并作为冷冻资源免费提供给研究界。从这一前所未有的资源中，IKMC与国际小鼠表型联盟(IMPC)合作产生了6000多个突变小鼠品系。此外，还建立了包括250株C57BL/6基因诱导小鼠的基因驱动源。作为对cre驱动资源的补充，还产生了一个由27个以组织特异性方式表达cre的raav组成的集合。所有资源都可以从IKMC/IMPC门户网站(www.mousephenotype.org)轻松访问。IKMC/IMPC资源是具有明确遗传背景的小鼠模型的标准化参考库，能够分析不同基因组成的小鼠的基因-疾病关联，因此应该对生物医学研究产生重大影响。

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来源期刊

Drug Discovery Today: Disease Models Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

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期刊介绍： Drug Discovery Today: Disease Models discusses the non-human experimental models through which inference is drawn regarding the molecular aetiology and pathogenesis of human disease. It provides critical analysis and evaluation of which models can genuinely inform the research community about the direct process of human disease, those which may have value in basic toxicology, and those which are simply designed for effective expression and raw characterisation.