Effects of bleomycin on kinetoplast DNA and nuclear DNA in trypanosoma gambiense using in situ microfluorometry technique

Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious... Pub Date : 1984-03-01 DOI:10.1016/S0174-3031(84)80006-2

Shozo Inoki , Humio Osaki , Masato Furuya

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引用次数: 2

Abstract

The effect of Bleomycin, an anti-tumour antibiotic, on either kinetoplast DNA(K-DNA) or nuclear DNA(N-DNA) in a single Trypanosoma gambiense cell was measured successfully utilizing the in situ microfluorometry technique. This microfluorometric assay clearly revealed that the relative fluorescence intensity (RFI) of K-DNA and N-DNA was greatly reduced by bleomycin, suggesting the occurrence of the single strand breaks in these DNAs. The present results have shown that bleomycin acts on N-DNA stronger than K-DNA at least in Trypanosoma gambiense. Microfluorometry technique can be employed valuably for studying the DNA damages of several anti-protozoan drugs.

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博来霉素对冈比亚锥虫动质体DNA和细胞核DNA的影响

利用原位微荧光技术成功地测定了抗肿瘤抗生素博来霉素对单个冈比亚锥虫细胞的着丝体DNA(K-DNA)或核DNA(N-DNA)的影响。微荧光分析清楚地显示，K-DNA和N-DNA的相对荧光强度(RFI)被博来霉素大大降低，表明这些dna中发生了单链断裂。目前的研究结果表明，至少在冈比亚锥虫中，博来霉素对N-DNA的作用强于K-DNA。微荧光技术在研究几种抗原生动物药物的DNA损伤方面具有重要的应用价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...

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