The Potential of Green Grape (Vitis vinifera L) Extract on Paraoxonase-3 Serum Levels in Rats were Given High Cholesterol Diet

Q3 Pharmacology, Toxicology and Pharmaceutics

Biomedical and Pharmacology Journal Pub Date : 2023-06-30 DOI:10.13005/bpj/2695

Ni Nyoman, A. Dewi, H. Bekti, Gusti Agung, Dewi Sarihati, Gusti Ayu Marhaeni, Luh Putu Rinawati

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引用次数: 0

Abstract

Grapes are rich in bioactive molecules that can act as antioxidants, antimicrobials, anti-inflammatory and anti-cancer. Paraoxonase (PON) is an enzyme that can combine with HDL and function as an antioxidant that can protect LDL and HDL from lipid peroxidation which can prevent atherosclerosis. We used a high-cholesterol diet given to Wistar Rats to find out the effect of green grape extract (GGE) on the production of PON3 serum levels in rats. GGE was made using the maceration method. Serum PON3 levels were measured using the ELISA method and measured at 450 nm. The results showed that the highest PON3 serum levels were found in the rat group which was given a GGE dose of 500 mg/200 g BW/day (AH2), followed by levels in rats given GGE at a concentration of 250 mg/200 g BW/day (AH1). Tamhane’s test showed that there was a difference between rats that were only given a high-cholesterol diet (DTK) and AH1, AH2, and rats that were fed a standard diet (DS) with AH1 and AH2. However, no difference was found between AH1 and AH2. The higher the dose of GGE given, the higher the serum PON3 level. Further research can better analyze the active compounds in GGE in increasing serum PON3 levels.

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绿葡萄提取物对高胆固醇大鼠血清对氧磷酶-3水平的影响

葡萄含有丰富的生物活性分子，可以作为抗氧化剂、抗菌剂、抗炎剂和抗癌剂。对氧磷酶(PON)是一种与高密度脂蛋白结合的酶，具有抗氧化剂的功能，可以保护低密度脂蛋白和高密度脂蛋白免受脂质过氧化，从而预防动脉粥样硬化。我们用高胆固醇饮食喂养Wistar大鼠，研究绿葡萄提取物(GGE)对大鼠血清PON3生成水平的影响。采用浸渍法制备GGE。采用ELISA法测定血清PON3水平，在450 nm处测定。结果显示，血清PON3水平以GGE剂量为500 mg/200 g BW/d组(AH2)最高，其次为GGE剂量为250 mg/200 g BW/d组(AH1)。Tamhane的实验表明，只喂食高胆固醇饮食(DTK)和AH1、AH2的大鼠与喂食含有AH1、AH2的标准饮食(DS)的大鼠之间存在差异。然而，AH1和AH2之间没有发现差异。GGE给药剂量越大，血清PON3水平越高。进一步的研究可以更好地分析GGE中有效成分对提高血清PON3水平的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical and Pharmacology Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

1.20

自引率

0.00%

发文量

189

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