Metabolite fingerprinting and profiling of selected medicinal plants using nuclear magnetic resonance

IF 0.2 Q4 PHARMACOLOGY & PHARMACY
Manas Sahoo, M. Umashankar
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Abstract

Background: Medicinal herbs are well known for their therapeutic effects and are traditionally used in the treatment and prevention of numerous diseases. Further plant-derived natural products have also been a valuable source of lead compounds for drug discovery and development. As the bioactivity of natural extracts is due to synergism between hundreds of metabolites present in the plant extract, the complete metabolomic analysis can be used for the quality control of phytomedicine. The 1H-nuclear magnetic resonance (NMR) fingerprint of the herbal extract can be used as a promising approach for comprehensive analysis of secondary metabolites to obtain a holistic view. The 1H-NMR spectroscopy has the advantage that it requires little quantity of samples and simple sample preparation method. Objectives: The study aimed to study the secondary metabolites of seven selected herbs, namely Abies webbiana, Cuminum cyminum, Elettaria cardamomum, Zingiber officinale, Glycyrrhiza glabra, Piper longum, and Terminalia chebula. Materials and Methods: The secondary metabolites of the herbal extracts were studied by recording the 1H-NMR spectra using NMR spectrometer in suitable solvent. Results: The putative metabolites that have been identified were 4-methoxy quercetin, luteolin, cuminaldehyde, 1,8-cineole, elettarins, gingerol, shogaol, glycyrrhizin, liquiritigenin, glabridin, betulinic acid, oleanolic acid, arabinogalactan, chebulagic acid, and gallic acid. Conclusion: The study highlighted the potential of NMR metabolomics in the study of plant extracts and their application for the quality control of phytomedicine.
利用核磁共振技术鉴定药用植物的代谢物指纹图谱
背景:草药以其治疗效果而闻名,传统上用于治疗和预防许多疾病。此外,植物衍生的天然产物也已成为药物发现和开发先导化合物的宝贵来源。由于天然提取物的生物活性是由于植物提取物中存在的数百种代谢物之间的协同作用,因此完全代谢组学分析可用于植物药的质量控制。中药提取物的1h -核磁共振(NMR)指纹图谱是一种很有前途的方法,可用于对次生代谢物进行综合分析,从而获得一个整体的观点。1H-NMR谱法具有样品用量少、制备方法简单等优点。目的:研究冷杉、小茴香、豆蔻、生姜、甘草、长椒、板栗等7种中草药的次生代谢产物。材料与方法:采用核磁共振波谱仪在合适的溶剂中记录其次生代谢产物。结果:鉴定出的代谢产物为4-甲氧基槲皮素、木犀草素、孜然醛、1,8-桉树油脑、薄荷素、姜辣素、芦果醇、甘草酸、甘草素、光甘草定、白桦木酸、齐墩果酸、阿拉伯半胱甘肽、七脂酸、没食子酸。结论:本研究突出了核磁共振代谢组学在植物提取物研究及其在植物药质量控制中的应用潜力。
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