Suitability of Sugarcane Extract as a Local Extender and the Use of Either DMSO or Glycerol as Cryoprotectant for the Cryopreservation of the Banaba Native Breed Chicken (Gallus gallus domesticus) Semen

Abstract

The experiment was designed to test the suitability of sugarcane extract (SE) as a local extender for the cryopreservation of the Banaba native chicken semen by comparing the effects of different cryoprotective agent concentrations [i.e. glycerol and dimethyl sulfoxide (DMSO)] and thawing temperatures on the post-thaw semen quality. Twelve (12) 29-month-old Banaba native roosters served as semen donors. Only samples that passed the preliminary semen quality assessment of > 70% motility and morphology were further processed and analyzed using a computer- assisted semen analyzer (CASA). Pooled ejaculate samples were cryopreserved with SE extender containing 3, 5, 7, or 9% (T3%, T5%, T7%, and T9%) glycerol or DMSO concentration levels. The results showed that the inclusion level of cryoprotectants and thawing temperatures have no significant effect on the percent motile sperm, percent progressive sperm, percent normal morphology, and percent viability. The type of cryoprotectant used in cryopreservation of the semen had a significant effect on all the semen quality parameters. Semen cryopreserved with glycerol had significantly higher values compared to DMSO in all semen quality variables.