Towards targeting signalling networks by functional phosphoproteomics

Abstract

We present the development of Proteo-Mode, an instrument for automated, high-throughput preparation of phosphoproteins for proteomics analysis of complex cellular signalling networks involving multiple, time-dependent protein phosphorylation events. Proteo-Mode automates all steps in the network analysis of phosphoproteins by proteomics method. This enables the integrated response of complex cellular signalling networks to be analyzed in normal and abnormal (disease) states and provides new perspectives in targeting and evaluation of the effects of therapeutic compounds on such networks.