On the production of particulate organic carbon by heterotrophic processes in sea water

Deep Sea Research (1953) Pub Date : 1961-01-01 DOI:10.1016/0146-6313(61)90022-3

T.R. Parsons, J.D.H. Strickland

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引用次数: 250

Abstract

It is suggested that the amount of heterotrophic carbon assimilation by micro-organisms in the sea may be a significant fraction of the photosynthetic production, when considering the whole water column in a deep ocean.

A method is described, allied to the carbon-14 method for measuring photosynthesis, whereby the heterotrophic uptake of any chosen substrate in sea water can be measured using the substrate labelled by carbon-14. The method is rapid and convenient and can give values of ‘relative-heterotrophic potential’. As with the radiochemical method for measuring marine photosynthesis, the exact interpretation of results presents many problems but the technique should prove a useful exploratory tool. Tests have been made using fully labelled acetate and glucose.

By a method of progressive radio-isotopic dilution it is possible to measure either the concentration or organic substrate in sea water or obtain information of the enzyme kinetics of the microorganisms assimilating a given substrate. A description is given of this method. A few preliminary trials to test its applicability have been encouraging.

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海水中异养过程产生颗粒有机碳的研究

这表明，当考虑到深海的整个水柱时，海洋中微生物的异养碳同化量可能占光合作用产量的很大一部分。本文描述了一种与测量光合作用的碳-14方法相关的方法，即可以使用碳-14标记的底物来测量海水中任何选定底物的异养吸收量。该方法快速、方便，能给出“相对异养电位”值。与测量海洋光合作用的放射化学方法一样，对结果的准确解释存在许多问题，但该技术应被证明是一种有用的探索工具。使用完全标记的醋酸盐和葡萄糖进行了测试。通过放射性同位素逐步稀释的方法，可以测量海水中有机底物的浓度或获得微生物同化给定底物的酶动力学的信息。对这种方法作了说明。一些测试其适用性的初步试验令人鼓舞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Deep Sea Research (1953)

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