Pollen grains and seed-morphology as related to Biochemical Patterns in five species of genus Ocimum L (Lamiaceae Juss.) of Saudi Arabia

W. T. Kasem
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引用次数: 2

Abstract

This study aims to investigate the pollen and seed characters by light microscopy (LM) and scanning electron microscope (SEM) as related to biochemical data of seed protein and esterase isoenzymes by SDS-PAGE technique in the five Saudi Arabian Ocimum L. species. The detailed description for pollen and seed in addition electrophoretic patterns were conducted by means of numerical analyses based on thirty-nine parameters. The pollen grains were zonocolpate, hexacolpate, prolate to subprolate. Three types of exine ornamentation were recognized, perforate, reticulate and granulate. Also,  The periclinal, the anticlinal and the boundaries between cells are described by the aid of SEM which exhibited four main distinct types of nutlets sculpture undulate, quirky, circular and straight. Molecular patterns of protein profiles and esterase (EC.3.1.1.1) showed that esterase could be considered as positive markers than protein, minimum and maximum gene / gene expression of esterase isoenzymes are demonstrated. The relationships between the studied taxa were demonstrated as a phenogram.
沙特阿拉伯五种茴香属植物的花粉粒和种子形态与生物化学模式的关系
本研究利用SDS-PAGE技术,利用光镜和扫描电镜分析了沙特阿拉伯五种酢浆草属植物的花粉和种子性状,以及种子蛋白和酯酶同工酶的生化数据。采用基于39个参数的数值分析方法对花粉和种子的附加电泳模式进行了详细描述。花粉粒呈带形、六形、长形至近长形。外壁纹饰分为三种类型:穿孔型、网状型和粒状型。此外,通过扫描电镜对细胞的周斜、背斜和边界进行了描述,显示出四种不同类型的小坚果雕塑:波浪形、古怪形、圆形和直形。蛋白谱和酯酶(EC.3.1.1.1)的分子图谱显示,酯酶可作为阳性标记物,而蛋白、酯酶同工酶的基因/基因表达量最小和最大。所研究的分类群之间的关系用物候图表示。
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