Optimization of sugar utilization by Azotobacter chroococcum in large scale fermentation for biomass production using response surface methodology

Abstract

A statistical model was developed in this study to describe biomass production through a fermentation process of . Response surface methodology (RSM) based on central composite CCD was employed to statistically evaluate and optimize the conditions for maximum biomass production and study the significance and interaction of Sucrose, KHPO and FeSO concentration of Jensson’s media on production of biomass. In the current study biomass optimize up to 13.4 g sucrose from 20g, KHPO 1.05g and FeSO 0.14g which give same biomass of . Thereby, sucrose cons different 6.6g in one liter, while compare with economic production cost at prize different Rs 4.3.00 for one liter. Also, for the production in e 300 liter fermenter Sucrose prize different was Rs 1292.00 per batch. Thereby, study has significantly optimize for quality purpose as well as prove to be economic.