External quality assessment for dual detection of HBsAg and anti-HCV in serum

Q4 Pharmacology, Toxicology and Pharmaceutics

Pharmaceutical Sciences Asia Pub Date : 2022-01-01 DOI:10.29090/psa.2022.01.21.09

Nguyen Thi Minh Thuan, Le Tran Bao Uyen, Le Chau Hoang Quoc Chuong, T. Ton

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Abstract

Hepatitis is a worldwide health problem leading to liver dysfunction, hepatocellular cirrhosis and carcinoma. Hepatitis B caused by the Hepatitis B virus (HBV) is known as a silent disease. Children infected with HBV often have no symptoms, making it difficult to monitor this population. Vietnam is a country with high rates of HBV and Hepatitis C virus (HCV) infection. Many people who have been infected with HBV and HCV without symptoms for a long time. They even do not know that they got infected, and this may risk infecting others. Therefore, HBV and HCV infection screening to prevent infection and disease progression is essential. Currently, HBsAg and anti-HCV assays have been performed in most laboratories using a variety of analytical methods with different biological products for HBV and HCV screening such as rapid tests (RTs), electroluminescence immunoassay (ECLIAs, chemiluminescent immunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA). Rapid test is a rapid chromatographic immunoassay for the detection of HBV and HCV in serum or plasma samples. Electroluminescence (ECL) assay is a technique for converting electrical energy into radiant energy called luminescence. Immunoassays (IAs) are analytical methods based on the antigen-antibody reactions for quantitative or qualitative analysis. The sensitivity and specificity of the ECLIA technique for the anti-HCV detection were 100% and 99.8%, respectively. ELISA is a labeled immunoassay and less sensitive than ECLIAs for HBsAg detection (73% compared to 100%). CLIA is an immunoassay technique using

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肝炎是一种全球性的健康问题，可导致肝功能障碍、肝细胞性肝硬化和肝癌。由乙型肝炎病毒(HBV)引起的乙型肝炎是一种无声的疾病。感染乙肝病毒的儿童通常没有症状，因此很难监测这一人群。越南是一个乙型肝炎病毒和丙型肝炎病毒(HCV)感染率很高的国家。许多长期感染HBV和HCV的人没有症状。他们甚至不知道自己被感染了，这可能会有感染他人的风险。因此，筛查HBV和HCV感染以预防感染和疾病进展至关重要。目前，大多数实验室使用不同生物制品的多种分析方法进行HBsAg和抗HCV检测，用于HBV和HCV筛查，如快速检测(RTs)、电致发光免疫测定(ECLIAs)、化学发光免疫测定(CLIA)和酶联免疫吸附测定(ELISA)。快速检测是一种快速色谱免疫分析法，用于检测血清或血浆样品中的HBV和HCV。电致发光(ECL)分析是一种将电能转化为称为发光的辐射能的技术。免疫测定法(IAs)是一种基于抗原-抗体反应的定量或定性分析方法。ECLIA技术检测抗- hcv的敏感性为100%，特异性为99.8%。ELISA是一种标记免疫测定法，对HBsAg检测的敏感性低于eclia(73%对100%)。CLIA是一种免疫分析技术

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来源期刊

Pharmaceutical Sciences Asia Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： The Pharmaceutical Sciences Asia (PSA) journal is a double-blinded peer-reviewed journal in English published quarterly, by the Faculty of Pharmacy, Mahidol University, Thailand. The PSA journal is formerly known as Mahidol University Journal of Pharmaceutical Sciences and committed to the timely publication of innovative articles and reviews. This journal is available in both printed and electronic formats. The PSA journal aims at establishing a publishing house that is open to all. It aims to disseminate knowledge; provide a learned reference in the field; and establish channels of communication between academic and research expert, policy makers and executives in industry and investment institutions. The journal publishes research articles, review articles, and scientific commentaries on all aspects of the pharmaceutical sciences and multidisciplinary field in health professions and medicine. More specifically, the journal publishes research on all areas of pharmaceutical sciences and related disciplines: Clinical Pharmacy Drug Synthesis and Discovery Targeted-Drug Delivery Pharmaceutics Biopharmaceutical Sciences Phytopharmaceutical Sciences Pharmacology and Toxicology Pharmaceutical Chemistry Nutraceuticals and Functional Foods Natural Products Social, Economic, and Administrative Pharmacy Clinical Drug Evaluation and Drug Policy Making Antimicrobials, Resistance and Infection Control Pharmacokinetics and Pharmacodynamics.