Evaluation of the Impact of Crude Oil Contamination on Soil's Physicochemical Characteristics, Micro-flora and Crop Yield

U. Okafor
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引用次数: 1

Abstract

The effects of crude oil pollution on soil physicochemical properties, microflora, and ecotoxicity were evaluated. Soil samples were contaminated with crude oil, and the effects of contamination on the physicochemical parameters, microflora, and growth index of bean (Phaseolus vulgaris) seeds were studied over a 6-month period. The heterotrophic bacteria isolated from the uncontaminated soil were Micrococcus, Klebsiella, Flavobacterium, Bacillus, Pseudomonas, and Serratia species, and the moulds included microbes such as Aspergillus niger, Fusarium, and Mucor sp. Petroleum contamination increased the pH of the soils to alkaline values while increasing the total nitrogen, organic carbon, and phosphorus contents. Electrical conductivity, nitrogen content, and phosphorus content were significantly reduced after petroleum contamination (p ˂ 0.05). The heavy metal contents of the contaminated soils decreased with increasing remediation time. Zinc, total nitrogen, total organic carbon, and electrical conductivity contents were statistically significantly different among samples throughout the bioremediation period (p ˂ 0.05). The ability of isolates to utilise hydrocarbons was highest for Pseudomonas and Bacillus species and lowest for Klebsiella and Serratia species. After a germination period of 12 days, a germination test showed that the bioattenuated polluted soil improved germination of bean seeds. Bioattenuation methods should be used and improved as a means of remediating petroleum-polluted sites because they are cost-effective and environmentally friendly.
原油污染对土壤理化特性、微生物区系及作物产量影响的评价
评价了原油污染对土壤理化性质、微生物区系和生态毒性的影响。采用原油污染土壤样品,研究了原油污染对菜豆(Phaseolus vulgaris)种子理化参数、菌群和生长指标的影响。从未污染的土壤中分离到的异养细菌有微球菌、克雷伯氏菌、黄杆菌、芽孢杆菌、假单胞菌和沙雷氏菌等,霉菌有黑曲霉、镰刀菌和毛霉等微生物。石油污染使土壤pH值升高至碱性,总氮、有机碳和磷含量升高。电导率、氮含量和磷含量在石油污染后显著降低(p小于0.05)。污染土壤重金属含量随修复时间的延长而降低。锌、总氮、总有机碳和电导率含量在整个生物修复期内差异有统计学意义(p小于0.05)。菌株利用碳氢化合物的能力在假单胞菌和芽孢杆菌中最高,在克雷伯氏菌和沙雷氏菌中最低。发芽期12 d后的发芽试验表明,生物衰减污染土壤提高了豆类种子的发芽率。生物衰减法作为修复石油污染场地的一种手段应加以使用和改进,因为它们具有成本效益和环境友好性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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