Medicinal Chemical Biology

Abstract

activity genetic profiling of resistant mutants that emerged following prolonged incubation of the lead compound with replicon cells A biotinylated derivative was used to affinity isolate NS5A, and streptavidin-mediated enrichment was competed by the presence of the parent compound confirm the specificity of target engagement. This work led to the development of the drug daclatasvir, an NS5A inhibitor for the treatment of HCV To further validate