17α-Methyltestosterone enhances growth and reproductive performance of immature male Asian catfish (Clarias macrocephalus)

Abstract

This study aimed to assess the effects of 17α-methyltestosterone (MT) on the growth and reproductive performance of male Asian catfish (Clarias macrocephalus). A total of 36 immature male Asian catfish (ABW = 125.97 g) were randomly distributed into 12 circular tanks, and fed with either the control diet (with no MT) or diets containing MT at 60 mg·kg-1, 90 mg·kg-1, or 120 mg·kg-1 for 45 days. Results showed that weight gain (WG), specific growth rate (SGR) and feed intake (FI) were significantly higher in the male catfish fed with diets containing 60 and 90 mg·kg-1 MT than in those fed with the control diet. Also, the diet attractability test revealed that the 60 and 90 mg·kg-1 MT groups significantly attracted more catfish than did the control diet; however, increasing MT to 120 mg·kg-1 reduced its attractability to the immature male catfish. MT-treated male catfish exhibited significantly heavier and significantly longer testes than male catfish fed the control diet at the termination of the feeding trial. These male catfish also exhibited significantly higher gonadosomatic indices (GSI) than catfish in the control group. Following induced spawning of nontreated female catfish and artificial fertilization of its eggs using testis preparation from the experimental male catfish at the termination of the feeding trial, results showed that testis preparations from all MT-treated males resulted in significantly higher fertilization (FR) and hatching rates (HR) of the eggs. In conclusion, incorporating MT to the diet improved both the growth and reproductive performance of the male Clarias macrocephalus. Precisely, dosages of optimal dietary MT using a quadratic model for maximal SGR, GSI, FR, and HR values were estimated to be 58.3, 75.0, 90.6, and 78.2 mg·kg-1, respectively.