GpIIb/IIIa is the main receptor for initial platelet adhesion to glass and titanium surfaces in contact with whole blood.

M. Broberg, C. Eriksson, H. Nygren
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引用次数: 54

Abstract

Platelets are the first cells to adhere to a surface in contact with blood and are capable of mediating several different responses after contact with different protein-coated surfaces. They are the main source of growth factors such as platelet-derived growth factor and are therefore important in the healing process. In this study, initial platelet adhesion to and spread on hydrophilic and hydrophobic (methylized) glass and titanium with similar wettability were investigated. Whole coagulating blood was used to simulate the in vivo situation shortly after implantation, in which bleeding precedes inflammation and wound healing. Several different antibodies directed against platelet integrins and receptors (CD9, FcgammaRII, GPIIb/IIIa, vitronectin receptor, GPIb/V/IX) were used in an attempt to block platelet adhesion to the surfaces. Immunofluorescence results show that initial platelet adhesion to all the surfaces we investigated can be almost completely inhibited (approximately 95%) by clone M148, an antibody against the GPIIb/IIIa complex (integrin alpha(IIb)beta(3); CD41/CD61), but not with other antibodies to the separate parts of the integrin. Antibodies known to inhibit fibrinogen binding to GPIIb/IIIa after adenosine diphosphate- and collagen- induced aggregation had very little effect on initial platelet adhesion. None of the other integrins were found to have such an effect on initial platelet adhesion. Antibody clone M148 was furthermore found to inhibit platelet spreading. This study shows that regardless of wettability and the biomaterial used, initial adhesion of platelets appears to be mediated by GPIIb/IIIa binding to surface adsorbed fibrinogen.
GpIIb/IIIa是与全血接触的玻璃和钛表面初始血小板粘附的主要受体。
血小板是第一个附着在与血液接触的表面上的细胞,并且在与不同的蛋白质包裹表面接触后能够介导几种不同的反应。它们是血小板衍生生长因子等生长因子的主要来源,因此在愈合过程中很重要。在本研究中,研究了具有相似润湿性的亲水性、疏水性(甲基化)玻璃和钛的初始血小板粘附和扩散。用全凝血模拟植入后不久的体内情况,在这种情况下,出血先于炎症和伤口愈合。几种不同的针对血小板整合素和受体(CD9, FcgammaRII, GPIIb/IIIa,玻璃体连接素受体,GPIb/V/IX)的抗体被用来阻止血小板粘附在表面。免疫荧光结果显示,我们研究的所有表面的初始血小板粘附几乎可以被克隆M148完全抑制(约95%),M148是一种针对GPIIb/IIIa复合物(整合素α (IIb) β)的抗体(3);CD41/CD61),但不与其他针对整合素单独部分的抗体结合。已知在二磷酸腺苷和胶原诱导聚集后抑制纤维蛋白原与GPIIb/IIIa结合的抗体对初始血小板粘附的影响很小。没有发现其他整合素对初始血小板粘附有这样的影响。抗体克隆M148进一步发现抑制血小板扩散。该研究表明,无论润湿性和使用的生物材料如何,血小板的初始粘附似乎是由GPIIb/IIIa与表面吸附的纤维蛋白原结合介导的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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