INCREASING OF THE SUPEROXIDE DISMUTASE TOTAL ACTIVITY IN MICROGLIAL CELLS UNDER THE TREATMENT BY $RIBES$ $NIGRUM$ L. ALCOHOL EXTRACT

N. Sahakyan, M. Petrosyan, A. Trchounian
{"title":"INCREASING OF THE SUPEROXIDE DISMUTASE TOTAL ACTIVITY IN MICROGLIAL CELLS UNDER THE TREATMENT BY $RIBES$ $NIGRUM$ L. ALCOHOL EXTRACT","authors":"N. Sahakyan, M. Petrosyan, A. Trchounian","doi":"10.46991/pysu:b/2020.54.3.216","DOIUrl":null,"url":null,"abstract":"Cell redox homeostasis is formed as a result of the balance between the accumulation of reactive oxygen species and functioning of the antioxidant enzymes or non-enzymatic antioxidants. The alcohol extracts from the leaves of Ribes nigrum L. were prepared to investigate the antioxidant activity and protective effects against both BV-2 microglial wild type (WT) cells and acyl-CoA oxidase type 1 (ACOX1) deficient cell (Acox1–/–) lines․ Our investigations showed that R. nigrum extracts possess remarkable antiradical activity in 1-diphenyl-2-picryl­hydrazyl (DPPH) with IC50 value of 32.7±0.8 µg mL–1. 3-(4,5-dimethyltrazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the sub-cytotoxic concentration of R. nigrum extract in both cases was 1 mg mL–1 The further treatment of microglial cells with the mentioned concentration of extract brought the remarkable increasing (up to 350%) of activity of the total superoxide dismutase (SOD) – the core antioxidant enzymes, under the treatment during the 48–72 h. So, relaying on the obtained data it is possible to insist that the lack of ACOX1 can be compensated by the activation of cell enzymatic antioxidant defense mechanisms.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the YSU B: Chemical and Biological Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.46991/pysu:b/2020.54.3.216","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2

Abstract

Cell redox homeostasis is formed as a result of the balance between the accumulation of reactive oxygen species and functioning of the antioxidant enzymes or non-enzymatic antioxidants. The alcohol extracts from the leaves of Ribes nigrum L. were prepared to investigate the antioxidant activity and protective effects against both BV-2 microglial wild type (WT) cells and acyl-CoA oxidase type 1 (ACOX1) deficient cell (Acox1–/–) lines․ Our investigations showed that R. nigrum extracts possess remarkable antiradical activity in 1-diphenyl-2-picryl­hydrazyl (DPPH) with IC50 value of 32.7±0.8 µg mL–1. 3-(4,5-dimethyltrazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the sub-cytotoxic concentration of R. nigrum extract in both cases was 1 mg mL–1 The further treatment of microglial cells with the mentioned concentration of extract brought the remarkable increasing (up to 350%) of activity of the total superoxide dismutase (SOD) – the core antioxidant enzymes, under the treatment during the 48–72 h. So, relaying on the obtained data it is possible to insist that the lack of ACOX1 can be compensated by the activation of cell enzymatic antioxidant defense mechanisms.
枸杞醇提取物对小胶质细胞超氧化物歧化酶总活性的影响
细胞氧化还原稳态是活性氧积累与抗氧化酶或非酶抗氧化剂功能之间的平衡的结果。摘要本实验研究了黑荆叶醇提物对BV-2小胶质野生型(WT)细胞和酰基辅酶a氧化酶1型(ACOX1 - / -)细胞的抗氧化活性和保护作用。结果表明,黑荆叶醇提物对1-二苯基-2-苦酰肼(DPPH)具有显著的抗自由基活性,IC50值为32.7±0.8µg mL-1。3-(4,5-二甲基曲唑-2-酰基)-2,5-二苯基溴化四唑(MTT)试验结果表明,两种情况下,黑荆提取物的亚细胞毒浓度均为1 mg mL-1。用上述浓度的提取物进一步处理小胶质细胞,在48-72 h内,其核心抗氧化酶总超氧化物歧化酶(SOD)的活性显著提高(高达350%)。根据获得的数据,有可能坚持认为ACOX1的缺乏可以通过激活细胞酶抗氧化防御机制来补偿。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信